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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-30113 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P2Y2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain, rat brain.
- Concentration
- 0.34 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of P2Y2 using 30 µg of A) 293T and B) A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a P2Y2 polyclonal antibody (Product # PA5-30113) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of P2Y2 was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a P2Y2 Polyclonal Antibody (Product # PA5-30113) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using P2Y2 Polyclonal Antibody (Product # PA5-30113). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 Polyclonal Antibody (Product # PA5-30113) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-P2Y2 Polyclonal Antibody (Product # PA5-30113) and a 42kDa band corresponding to P2Y2 was observed across the cell lines tested. Membrane extracts (30ug lysate) of HL-60 (Lane 1), THP-1 (Lane 2), PC-3 (Lane 3), HAEC (Lane 4) and MCF7 (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of P2Y2 was performed in DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: P2Y2 Polyclonal Antibody (Product # PA5-30113) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of P2Y2 was performed in 293T whole cell extracts using 5 µg of P2Y2 Polyclonal Antibody (Product # PA5-30113). Samples were transferred to a membrane and probed with P2Y2 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
