APR-106-200UL

antibody from Invitrogen Antibodies

Targeting: P2RY6 P2Y6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

APR-106-200UL

Provider

Invitrogen Antibodies

Product name

P2Y6 Receptor (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)heart membranes: - 1,2. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:400).3,4. Anti-P2Y6 Receptor (extracellular) Antibody , preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1,2. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:200).3,4. Anti-P2Y6 Receptor (extracellular) Antibody , preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)heart membranes: - 1,2. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:400).3,4. Anti-P2Y6 Receptor (extracellular) Antibody , preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1,2. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:200).3,4. Anti-P2Y6 Receptor (extracellular) Antibody , preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate: - 1. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:200). 2. Anti-P2Y6 Receptor (extracellular) Antibody , preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of P2RY6 in rat parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:1000), followed by donkey- Anti-rabbit-biotin and streptavidin-Cy3. P2RY6 immunoreactivity (red) appears in pyramidal neurons (arrows). Cell nuclei are stained with DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of P2RY6 live intact mouse J774 macrophage cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (2.5 µg) + goat- Anti-rabbit-FITC.