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Learn210-501-B32
antibody from Rockland Immunochemicals, Inc.
Targeting: IL17A
CTLA8, IL-17, IL-17A, IL17
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 210-501-B32 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#210-501-B32, RRID:AB_11179931
- Product name
- Anti-Mouse IL-17A (RAT) Monoclonal Antibody - 210-501-B32
- Antibody type
- Monoclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot showing detection of Mouse IL-17A. 100 ng of Mouse IL-17A was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C, Anti-Mouse IL-17A (RAT) Antibody (p/n 210-501-B32) was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody (p/n 610-4302) was diluted in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A.
- Validation comment
- Western Blot
