TA501287

antibody from OriGene

Targeting: USP5 IsoT

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501287

Provider

OriGene

Proper citation

OriGene Cat#TA501287, RRID:AB_11125013

Product name

Anti-USP5 mouse monoclonal antibody, clone OTI1F8 (formerly 1F8)

Antibody type

Monoclonal

Description

Anti-USP5 mouse monoclonal antibody, clone OTI1F8 (formerly 1F8)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

USP5

Isotype

IgG

Antibody clone number

OTI1F8

Vial size

100 µl

Concentration

0.7 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP5 (RC202624, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP5 monoclonal antibody.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501287, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501287, Dilution 1:50)

Validation comment

IHC