16431-1-AP

antibody from Proteintech Group

Targeting: DLD DLDH, E3, GCSL, LAD, OGDC-E3

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16431-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16431-1-AP, RRID:AB_2091888

Product name

DLD antibody

Antibody type

Polyclonal

Description

DLD antibody (Cat. #16431-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

DLD, upregulated by YY1, fuels glioblastoma multiforme progression via EphA2 phosphorylation to activate PI3K/AKT/mTOR.

Tang G, Zhou Y, Su J, Yuan M, You J, Huang W, Li H, Zhao Z
QJM : monthly journal of the Association of Physicians 2026 Mar 9;

---

Mitoregulin supports mitochondrial membrane integrity and protects against cardiac ischaemia-reperfusion injury.

Stein CS, Zhang X, Witmer NH, Pennington ER, Hahn S, Straub AC, Shaikh SR, Boudreau RL
Cardiovascular research 2026 Mar 26;122(3):379-396

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RBM15 Mediated m6A Modification of SRSF1 Inhibits Cuproptosis in Non-Small Cell Lung Cancer by Mediating ATP7B Alternative Splicing.

Mao SS, Wu DY, Cui RH, Cheng XZ
The Kaohsiung journal of medical sciences 2026 Jan;42(1):e70098

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Bioinformatics and clinical analysis reveal the potential diagnostic and prognostic role of DLD for pan cancer and renal carcinoma.

Sun F, Zhang D, Ma X, Wang L, Ma J, Wu J
Discover oncology 2026 Feb 17;17(1):328

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Altered astrocyte-neuron crosstalk in progressive supranuclear palsy: integrated evidence from proteomics and magnetic resonance spectroscopy.

Ono M, Kumagai Y, Hirata K, Maeda Y, Abe Y, Endo H, Shimizu H, Kakita A, Uchida Y, Takado Y
Acta neuropathologica 2026 Apr 24;151(1)

---

Dexmedetomidine alleviates lung ischemia-reperfusion injury by inhibiting cuproptosis: an in vivo study.

Liu H, Wang Z, Qiu T, Du W, Wu Z, Chen S, Jiao W
Frontiers in pharmacology 2025;16:1562535

---

Non-canonical dihydrolipoyl transacetylase promotes chemotherapy resistance via mitochondrial tetrahydrofolate signaling.

Hwang JS, Kang J, Kim J, Eun K, West S, Bacho HE, Avalos V, Shuff S, Shin DM, Saba NF, Magliocca KR, Qu CK, Fu H, Ramalingam SS, Ivanov AA, Hitosugi T, Kang S
Nature communications 2025 Oct 8;16(1):8932

---

Mitochondrial NADPH fuels mitochondrial fatty acid synthesis and lipoylation to power oxidative metabolism.

Kim D, Kesavan R, Ryu K, Dey T, Marckx A, Menezes C, Praharaj PP, Morley S, Ko B, Soflaee MH, Tom HJ, Brown H, Vu HS, Tso SC, Brautigam CA, Lemoff A, Mettlen M, Mishra P, Cai F, Allen DK, Hoxhaj G
Nature cell biology 2025 May;27(5):790-800

---

Targeting FDX1 by trilobatin to inhibit cuproptosis in doxorubicin-induced cardiotoxicity.

Wei J, Lan G, Zhang W, Ran W, Wei Y, Liu X, Zhang Y, Gong Q, Li H, Gao J
British journal of pharmacology 2025 Jun;182(11):2409-2425

---

Diallyl Trisulfide From Garlic Regulates RAB18 Phase Separation to Inhibit Lipophagy and Induce Cuproptosis in Hepatic Stellate Cells for Antifibrotic Effects.

Tian H, Sun S, Qiu X, Wang J, Gao Y, Chen J, Han X, Bao Z, Guo X, Sun Y, Lin Y, Hu M, Zhang F, Zhang Z, Wang F, Zheng S, Shao J
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jun;12(21):e2415325

---

Opposing effects of systemic and pancreas-specific inhibition of BCKDK on pancreatic carcinogenesis.

Noji MC, Demetriadou C, Landis M, Pennise J, Pinheiro LV, Jaccard A, Chatoff A, Drummond J, Guo K, Azor R, Robertson MR, Kawakami R, Marcinkiewicz MM, Tobias JW, Snyder NW, Feldser D, Arany Z, Wellen KE
bioRxiv : the preprint server for biology 2025 Jun 15;

---

Dysregulation of mitochondrial α-ketoglutarate dehydrogenase leads to elevated lipid peroxidation in CHCHD2-linked Parkinson's disease models.

Gao G, Shi Y, Deng HX, Krainc D
Nature communications 2025 Feb 26;16(1):1982

---

Targeting multiple genetic defects of mitochondrial diseases with a single bacterial lipoate protein ligase.

Hu Z, Yu J, Liu Z, Jiang M, Zeng AP
Science advances 2025 Dec 5;11(49):eaea8481

---

Deep Mutational Scanning of FDX1 Identifies Key Structural Determinants of Lipoylation and Cuproptosis.

Hsiao JC, Warui DM, Kwon JJ, Dreishpoon MB, Bick NR, Blue-Lahom T, Root DE, Booker SJ, Tsvetkov P
Nature communications 2025 Dec 21;17(1):1112

---

Genome-Wide Mendelian Randomization Identifies Ferroptosis-Related Drug Targets for Alzheimer's Disease.

Wang Y, Song X, Wang R, Xu X, Du Y, Chen G, Mei J
Journal of Alzheimer's disease reports 2024;8(1):1185-1197

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Construction and validation of a bioinformatics‑based screen for cuproptosis‑related genes and risk model for Alzheimer's disease.

Hu R, Xiao Z, Qiao M, Liu C, Wu G, Wang Y, Dong M, Huang Z
Molecular medicine reports 2024 Nov;30(5)

---

NOTCH1 mitochondria localization during heart development promotes mitochondrial metabolism and the endothelial-to-mesenchymal transition in mice.

Wang J, Zhao R, Xu S, Zhou XY, Cai K, Chen YL, Zhou ZY, Sun X, Shi Y, Wang F, Gui YH, Tao H, Zhao JY
Nature communications 2024 Nov 16;15(1):9945

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Clinical features and molecular landscape of cuproptosis signature-related molecular subtype in gastric cancer.

Chong W, Ren H, Chen H, Xu K, Zhu X, Liu Y, Sang Y, Li H, Liu J, Ye C, Shang L, Jing C, Li L
iMeta 2024 Jun;3(3):e190

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CPT1A loss disrupts BCAA metabolism to confer therapeutic vulnerability in TP53-mutated liver cancer.

Liu Y, Wang F, Yan G, Tong Y, Guo W, Li S, Qian Y, Li Q, Shu Y, Zhang L, Zhang Y, Xia Q
Cancer letters 2024 Jul 28;595:217006

---

Molecular characterization and clinical relevance of metabolic signature subtypes in gastric cancer.

Chen H, Jing C, Shang L, Zhu X, Zhang R, Liu Y, Wang M, Xu K, Ma T, Jing H, Wang Z, Li X, Chong W, Li L
Cell reports 2024 Jul 23;43(7):114424

---

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation.

Mao Y, Zhang J, Zhou Q, He X, Zheng Z, Wei Y, Zhou K, Lin Y, Yu H, Zhang H, Zhou Y, Lin P, Wu B, Yuan Y, Zhao J, Xu W, Zhao S
Cell research 2024 Jan;34(1):13-30

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A cuproptosis-related prognostic signature for guiding clinical diagnosis and treatment in uveal melanoma patients.

Yang Y, Li Q, Chen J, Guo Y, Cai Y, Zhao W, Su S, Sang A
Heliyon 2024 Aug 30;10(16):e36324

---

Dihydrolipoamide dehydrogenase (DLD) is a novel molecular target of bortezomib.

Feng Y, Luo H, Huang J, Zhang Y, Wen J, Li L, Mi Z, Gao Q, He S, Liu X, Zhai X, Wang X, Zhang L, Niu T, Zheng Y
Cell death & disease 2024 Aug 13;15(8):588

---

Monocarboxylate transporter 4 deficiency enhances high-intensity interval training-induced metabolic adaptations in skeletal muscle.

Tamura Y, Jee E, Kouzaki K, Kotani T, Nakazato K
The Journal of physiology 2024 Apr;602(7):1313-1340

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Demystifying the landscape of endometrial immune microenvironment in luteal-phase from cuprotosis: Implications for the mechanism and treatment of RPL.

Zhao X, Wang S, Du T, Jiang Y, Zhao Y, Ma Y, Shen D, Shen Y, Ma J
Gene 2024 Apr 20;903:148191

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ITGB1-mediated molecular landscape and cuproptosis phenotype induced the worse prognosis in diffuse gastric cancer.

Zhu X, Chen H, Li H, Ren H, Ye C, Xu K, Liu J, Du F, Zhang Z, Liu Y, Xie X, Wang M, Ma T, Chong W, Shang L, Li L
Frontiers in oncology 2023;13:1115510

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Branched-chain amino acids promote thrombocytopoiesis by activating mTOR signaling.

Jiang H, Zhang L, Yang M, Li G, Ding C, Xin M, Dai J, Sun X, Fan X, Sun H, Liu J, Xu Y
Journal of thrombosis and haemostasis : JTH 2023 Nov;21(11):3224-3235

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Bioinformatics-Based Analysis of Key Genes in Steroid-Induced Osteonecrosis of the Femoral Head That Are Associated with Copper Metabolism.

Qi B, Li C, Cai X, Pu L, Guo M, Tang Z, Bu P, Xu Y
Biomedicines 2023 Mar 13;11(3)

---

Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress.

Liu Y, Liu K, Thorne RF, Shi R, Zhang Q, Wu M, Liu L
Cell reports 2023 Feb 28;42(2):112041

---

Oxoglutarate dehydrogenase-like inhibits the progression of hepatocellular carcinoma by inducing DNA damage through non-canonical function.

Jiang X, Peng J, Xie Y, Xu Y, Liu Q, Cheng C, Yan P, Xu S, Wang Y, Zhang L, Li H, Li Y, Li B, Han J, Yu D
Cell death and differentiation 2023 Aug;30(8):1931-1942

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Silencing of OGDHL promotes liver cancer metastasis by enhancing hypoxia inducible factor 1 α protein stability.

Dai W, Li Y, Sun W, Ji M, Bao R, Chen J, Xu S, Dai Y, Chen Y, Liu W, Ge C, Sun W, Mo W, Guo C, Xu X
Cancer science 2023 Apr;114(4):1309-1323

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A novel signature to guide osteosarcoma prognosis and immune microenvironment: Cuproptosis-related lncRNA.

Yang M, Zheng H, Xu K, Yuan Q, Aihaiti Y, Cai Y, Xu P
Frontiers in immunology 2022;13:919231

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AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis.

Wang Y, Luo M, Wang F, Tong Y, Li L, Shu Y, Qiao K, Zhang L, Yan G, Liu J, Ji H, Xie Y, Zhang Y, Gao WQ, Liu Y
Nature communications 2022 Nov 24;13(1):7215

---

Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin.

Head PE, Myung S, Chen Y, Schneller JL, Wang C, Duncan N, Hoffman P, Chang D, Gebremariam A, Gucek M, Manoli I, Venditti CP
Science translational medicine 2022 May 25;14(646):eabn4772

---

Copper induces cell death by targeting lipoylated TCA cycle proteins.

Tsvetkov P, Coy S, Petrova B, Dreishpoon M, Verma A, Abdusamad M, Rossen J, Joesch-Cohen L, Humeidi R, Spangler RD, Eaton JK, Frenkel E, Kocak M, Corsello SM, Lutsenko S, Kanarek N, Santagata S, Golub TR
Science (New York, N.Y.) 2022 Mar 18;375(6586):1254-1261

---

Proteomic analysis reveals the heterogeneity of metabolic reprogramming in lacrimal gland tumors.

Yang J, Li Y, Gao W, Feng Y, He X, Ni H, Song X, Fan J
Experimental eye research 2022 Jun;219:109052

---

Beneficial effects of aloperine on inflammation and oxidative stress by suppressing necroptosis in lipopolysaccharide-induced acute lung injury mouse model.

Cui YR, Qu F, Zhong WJ, Yang HH, Zeng J, Huang JH, Liu J, Zhang MY, Zhou Y, Guan CX
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jun;100:154074

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Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.

Peng MZ, Shao YX, Li XZ, Zhang KD, Cai YN, Lin YT, Jiang MY, Liu ZC, Su XY, Zhang W, Jiang XL, Liu L
Cellular and molecular life sciences : CMLS 2022 Jun 21;79(7):375

---

Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.

Xia J, Zhang J, Wu X, Du W, Zhu Y, Liu X, Liu Z, Meng B, Guo J, Yang Q, Wang Y, Wang Q, Feng X, Xie G, Shen Y, He Y, Xiang J, Wu M, An G, Qiu L, Jia W, Zhou W
Nature communications 2022 Jul 11;13(1):4007

---

Metabolon formation regulates branched-chain amino acid oxidation and homeostasis.

Patrick M, Gu Z, Zhang G, Wynn RM, Kaphle P, Cao H, Vu H, Cai F, Gao X, Zhang Y, Chen M, Ni M, Chuang DT, DeBerardinis RJ, Xu J
Nature metabolism 2022 Dec;4(12):1775-1791

---

Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways.

Chen Y, Zhang T, Zeng S, Xu R, Jin K, Coorey NJ, Wang Y, Wang K, Lee SR, Yam M, Zhu M, Chang A, Fan X, Zhang M, Du J, Gillies MC, Zhu L
Redox biology 2022 Aug;54:102379

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OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Dai W, Xu L, Yu X, Zhang G, Guo H, Liu H, Song G, Weng S, Dong L, Zhu J, Liu T, Guo C, Shen X
Journal of hepatology 2020 May;72(5):909-923

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Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.

Horita H, Law A, Middleton K
Proteomes 2018 May 22;6(2)

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RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis.

Yang Z, Wang Y, Zhang Y, He X, Zhong CQ, Ni H, Chen X, Liang Y, Wu J, Zhao S, Zhou D, Han J
Nature cell biology 2018 Feb;20(2):186-197

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Riboflavin responsive mitochondrial myopathy is a new phenotype of dihydrolipoamide dehydrogenase deficiency. The chaperon-like effect of vitamin B2.

Carrozzo R, Torraco A, Fiermonte G, Martinelli D, Di Nottia M, Rizza T, Vozza A, Verrigni D, Diodato D, Parisi G, Maiorana A, Rizzo C, Pierri CL, Zucano S, Piemonte F, Bertini E, Dionisi-Vici C
Mitochondrion 2014 Sep;18:49-57

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human liver tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP(DLD antibody) at dilution of 1:500

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 16431-1-AP(DLD antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The DLD antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human DLD. This antibody recognizes human,mouse,rat antigen. The DLD antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

16431-1-AP.pdf

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