R30535

antibody from NSJ Bioreagents

Targeting: DLD DLDH, E3, GCSL, LAD, OGDC-E3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R30535

Provider

NSJ Bioreagents

Product name

Lipoamide Dehydrogenase Antibody

Antibody type

Polyclonal

Antigen

An amino acid sequence from the C-terminus of human Lipoamide Dehydrogenase (EAFREANLAASFGKSINF) was used as the immunogen for this Lipoamide Dehydrogenase antibody.

Description

Antigen affinity purified antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Concentration

Lyophilized; resuspend with 200 ul for 0.5 mg/ml

Storage

After reconstitution, the Lipoamide Dehydrogenase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Lipoamide Dehydrogenase antibody and Lane 1: rat liver; 2: rat brain; 3: rat ovary; 4: rat testis; 5: SMMC-7721; 6: HeLa; 7: SMMC-7721; 8: Jurkat cell lysate

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human K562, 2) human A431, 3) human SiHa, 4) human U-251, 5) rat liver, 6) rat heart, 7) mouse liver and 8) mouse heart tissue lysate with Lipoamide Dehydrogenase antibody. Predicted molecular weight ~54 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ICC testing of MCF-7 cells

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ICC staining of FFPE human MCF7 cells with Lipoamide Dehydrogenase antibody. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human HCT116 cells with Lipoamide Dehydrogenase antibody. HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Lipoamide Dehydrogenase antibody testing of human breast cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat liver tissue with Lipoamide Dehydrogenase antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human breast cancer tissue with Lipoamide Dehydrogenase antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.