PA5-70397

antibody from Invitrogen Antibodies

Targeting: DLD DLDH, E3, GCSL, LAD, OGDC-E3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-70397

Provider

Invitrogen Antibodies

Product name

DLD Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This target displays homology in the following species: Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 93%; Yeast: 86%; Zebrafish: 93%

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition.

Mullen PJ, Garcia G Jr, Purkayastha A, Matulionis N, Schmid EW, Momcilovic M, Sen C, Langerman J, Ramaiah A, Shackelford DB, Damoiseaux R, French SW, Plath K, Gomperts BN, Arumugaswami V, Christofk HR
Nature communications 2021 Mar 25;12(1):1876

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DLD on human Jurkat cells. The sample was probed with a DLD polyclonal antibody (Product # PA5-70397) using a primary antibody dilution of 0.2-1.0 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of DLD in paraffin-embedded human pineal tissue. Sample was probed with a DLD polyclonal antibody (Product # PA5-70397) at a dilution of 1:100. Detection was performed with a donkey anti-Rabbit Cy3 secondary antibody at a dilution of 1:200. Images were taken at 20x magnification with an exposure time of 0.5-2.0 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 SARS-CoV-2 infection alters TCA cycle metabolism. a Glutamine entry into the TCA cycle is reduced in SARS-CoV-2 infected cells. Mass isotopomer analysis of TCA cycle metabolites in mock or SARS-CoV-2 infected Vero kidney epithelial cells after 24 h incubation with U- 13 C-glutamine. Each isotopomer is a different color, which is defined in the figure. P values compare the total fraction labeled between mock and SARS-CoV-2 infected cells ( n = 3 biologically independent samples). b The oxidative TCA cycle is decreased in SARS-CoV-2 infected (red bars) Vero cells after 24 h infection compared with mock infected (gray bars) Vero cells ( n = 3 biologically independent samples). c The reductive TCA cycle is maintained in SARS-CoV-2 infected (red bars) Vero cells compared with mock infected (gray bars) Vero cells after 24 h infection ( n = 3 biologically independent samples). d Levels of alphaKGC members OGDH and DLD are reduced in SARS-CoV-2 infected Vero cells (representative of two biological independent samples). e Glucose-derived carbon entry into the TCA cycle is increased in SARS-CoV-2 infected cells. Mass isotopomer analysis of TCA cycle metabolites in mock or SARS-CoV-2 infected Vero kidney epithelial cells after 24 h incubation with U- 13 C-glucose. Each isotopomer is a different color, which is defined in the figure. P values compare the total fraction labeled between mock and SARS-CoV-2 infected cells ( n = 3 biologically independent samples). f Glucose-derived c