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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27367 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DLD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, mouse brain, PC-12, Rat2. Predicted reactivity: Mouse (96%), Rat (96%), Xenopus laevis (94%), Dog (98%), Pig (99%), Chicken (93%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Identification of host proteins differentially associated with HIV-1 RNA splice variants.
Knoener R, Evans E 3rd, Becker JT, Scalf M, Benner B, Sherer NM, Smith LM
eLife 2021 Feb 25;10
eLife 2021 Feb 25;10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DLD was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a DLD Polyclonal Antibody (Product # PA5-27367) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DLD Polyclonal Antibody (Product # PA5-27367). Sample (30 µg of whole cell lysate). A: 293T. 10% SDS PAGE. DLD Polyclonal Antibody (Product # PA5-27367) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DLD Polyclonal Antibody (Product # PA5-27367). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. DLD Polyclonal Antibody (Product # PA5-27367) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of DLD was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: DLD Polyclonal Antibody (Product # PA5-27367) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using DLD (Product # PA5-27367) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
