Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-77103 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MBTPS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MBTPS1 in extracts of various cell lines. Samples were incubated with MBTPS1 polyclonal antibody (Product # PA5-77103).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MBTPS1 in extracts of various cell lines. Samples were incubated with MBTPS1 polyclonal antibody (Product # PA5-77103).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MBTPS1 Polyclonal Antibody (Product # PA5-77103) and a ~130kDa band corresponding to Membrane-bound transcription factor site-1 protease was observed across cell lines and tissues tested . Membrane enriched extracts (30 µg lysate) of SK-O-V3 (Lane 1), SK-O-V3 treated with 1X PTI for 4h (Lane 2), A549 (Lane 3), A549 treated with 1X PTI for 4h (Lane 4), MCF7 (Lane 5), MCF7 treated with 1X PTI for 4h (Lane 6), HeLa (Lane 7), U-87 MG (Lane 8) Mouse Liver (Lane 9), Mouse Spleen (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).A non specific band was observed around 42 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MBTPS1 in U20S cells. Samples were incubated with MBTPS1 polyclonal antibody (Product # PA5-77103) followed by DAPI (nuclear staining).