Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PB10085 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-PML Protein Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for Pml detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Mouse. Pml information: Molecular Weight: 98242 MW; Subcellular Localization: Nucleus . Nucleus, nucleoplasm. Cytoplasm. Nucleus, PML body. Nucleus, nucleolus . Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Detected in the nucleolus after DNA damage. Acetylation at Lys-497 is essential for its nuclear localization. Within the nucleus, most of PML is expressed in the diffuse nuclear fraction of the nucleoplasm and only a small fraction is found in the matrix-associated nuclear bodies (PML-NBs). The transfer of PML from the nucleoplasm to PML-NBs depends on its phosphorylation and sumoylation. The B1 box and the RING finger are also required for the localization in PML-NBs. Also found in specific membrane structures termed mitochondria-associated membranes (MAMs) which connect the endoplasmic reticulum (ER) and the mitochondria (By similarity).
- Reactivity
- Mouse
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of PML Protein expression in mouse testis extract (lane 1). PML Protein at 72KD, 97KD was detected using rabbit anti- PML Protein Antigen Affinity purified polyclonal antibody (Catalog # PB10085) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image