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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 21041-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PML Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LHTLCSGCL EASGMQCPIC QAPWPLGADT PALDNVFFES LQRRLSVYRQ IVDAQAVCTR CKESADFWCF ECEQLLCAKC FEAHQWFLKH EARPLAELRN QSVREFLDGT RKTNNIFCSN PNHRTPTLTS IYCRGCSKPL CCSCALLDSS HSDLKCDISA EIQQRQEELD AMTQALQEQD SAFGAVHAQM HAAVGQLGRA RAETEELIRE RVRQVVAHVR AQERELLEAV DARYQRDYEE MASRLGRLDA VLQRIRTGSA LVQRMKCYAS DQEVLDMHGF LRQALCRLRQ EEPQSLQAAV RTDGFDEFKV RLQDLSSCIT QGKDAAVSKK ASPEAASTPR DPIDVDL (73-418 aa encoded by BC020994)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.28 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 21041-1-AP ( PML Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of PML antibody (21041-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PML transfected HeLa cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 21041-1-AP (PML antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 21041-1-AP ( PML Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 21041-1-AP ( PML Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PML (IP:21041-1-AP, 4ug; Detection:21041-1-AP 1:500) with HEK-293 cells lysate 3200ug.
