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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-78299 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCLAF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: K562, K562 nuclear extract Predicted Reactivity: Pig (96%), Bovine (95%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BTF in K562 cells using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with BTF polyclonal antibody (Product # PA5-78299) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BCLAF1 Polyclonal Antibody (Product # PA5-78299). K562 whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BCLAF1 Polyclonal Antibody (Product # PA5-78299) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCLAF1 Polyclonal Antibody detects BTF protein at nucleus by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BTF protein stained by BCLAF1 Polyclonal Antibody (Product # PA5-78299) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCLAF1 Polyclonal Antibody detects BTF protein at nucleus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. BCLAF1 Polyclonal Antibody (Product # PA5-78299) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCLAF1 Polyclonal Antibody detects BTF protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. BCLAF1 Polyclonal Antibody (Product # PA5-78299) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
