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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00009260-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009260-M01, RRID:AB_437090
- Product name
- PDLIM7 monoclonal antibody (M01), clone 2D6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PDLIM7.
- Antigen sequence
MDSFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTP
GGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKI
RACGERLSLGLSRAQPVQSKPQKASAPAADPPRYT
FAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLV
PDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTG
TEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPW
PGPTAPSPTSRPPWAVDPAFAERYAPDKTSTVLTR
HSQPATPTPLQSRTSIVQAAAGGVPGGGSNNGKTP
VCHQCHKVIRGRYLVALGHAYHPEEFVCSQCGKVL
EEGGFFEEKGAIFCPPCYDVRYAPSCAKCKKKITG
EIMHALKMTWHVHCFTCAACKTPIRNRAFYMEEGV
PYCERDYEKMFGTKCHGCDFKIDAGDRFLEALGFS
WHDTCFVCAICQINLEGKTFYSKKDRPLCKSHAFS
HV- Isotype
- IgG
- Antibody clone number
- 2D6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Postsynaptic PDLIM5/Enigma Homolog binds SPAR and causes dendritic spine shrinkage.
Herrick S, Evers DM, Lee JY, Udagawa N, Pak DT
Molecular and cellular neurosciences 2010 Feb;43(2):188-200
Molecular and cellular neurosciences 2010 Feb;43(2):188-200
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PDLIM7 monoclonal antibody (M01), clone 2D6 Western Blot analysis of PDLIM7 expression in Hela S3 NE ( Cat # L013V3 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PDLIM7 expression in transfected 293T cell line by PDLIM7 monoclonal antibody (M01), clone 2D6.Lane 1: PDLIM7 transfected lysate(49.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PDLIM7 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PDLIM7 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PDLIM7 transfected lysate using anti-PDLIM7 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PDLIM7 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
