Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-53846 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDLIM7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: HLTGTEFMQD PDEEHLKKSS QVPRTEAPAP ASSTPQEPWP GPTAPSPTSR PPWAVDPAFA ERYAPDKTST VLTR Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 92%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDLIM7 in human cell lines U2OS and HEK293 using a PDLIM7 Polyclonal Antibody (Product # PA5-53846). Corresponding PDLIM7 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDLIM7 using PDLIM7 Polyclonal Antibody (Product # PA5-53846) (A) shows similar pattern to an independent PDLIM7 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PDLIM7 in human cell line U-251 MG shows positivity in actin filaments & focal adhesion sites. Samples were probed using a PDLIM7 Polyclonal Antibody (Product # PA5-53846).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of PDLIM7 in human cell line U-251 MG using PDLIM7 Polyclonal Antibody (Product # PA5-53846). Staining shows localization to actin filaments and focal adhesion sites.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PDLIM7 in human heart muscle using a PDLIM7 Polyclonal Antibody (Product # PA5-53846) shows moderate cytoplasmic positivity in myocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PDLIM7 in human heart muscle using a PDLIM7 Polyclonal Antibody (Product # PA5-53846) shows moderate cytoplasmic positivity in myocytes.