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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NBP2-25028 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal 53BP1 Antibody
- Antibody type
- Monoclonal
- Description
- Ammonium sulfate precipitation.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references TIN2 Functions with TPP1/POT1 To Stimulate Telomerase Processivity.
Defective DNA single-strand break repair is responsible for senescence and neoplastic escape of epithelial cells.
Pike AM, Strong MA, Ouyang JPT, Greider CW
Molecular and cellular biology 2019 Nov 1;39(21)
Molecular and cellular biology 2019 Nov 1;39(21)
Defective DNA single-strand break repair is responsible for senescence and neoplastic escape of epithelial cells.
Nassour J, Martien S, Martin N, Deruy E, Tomellini E, Malaquin N, Bouali F, Sabatier L, Wernert N, Pinte S, Gilson E, Pourtier A, Pluquet O, Abbadie C
Nature communications 2016 Jan 29;7:10399
Nature communications 2016 Jan 29;7:10399
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 53BP1 Antibody (6B3E10) [NBP2-25028] - Western blot analysis using 53BP1 Antibody against human 53BP1 recombinant protein. The expected molecular weight is 47.6 kDa which is demonstrated in this analysis, and the theoretical molecular weight of the whole endogenous protein is 214 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 53BP1 Antibody (6B3E10) [NBP2-25028] - Western blot analysis using against HEK293 (1) and 53BP1 (AA: 574-773)-hIgGFc transfected HEK293 (2) cell lysate. The observed molecular weight is ~52 kDa and the theoretical molecular weight of the whole endogenous protein is 214 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: 53BP1 Antibody (6B3E10) [NBP2-25028] - Analysis of paraffin-embedded endometrial cancer tissues using 53BP1 Antibody with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: 53BP1 Antibody (6B3E10) [NBP2-25028] - Analysis of paraffin-embedded colon cancer tissues using 53BP1 Antibody with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: 53BP1 Antibody (6B3E10) [NBP2-25028] - Analysis of HepG2 cells using 53BP1 Antibody (green) and negative control (purple).
