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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF3405 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal 53BP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human 53BP1 when phosphorylated at S25.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references 53BP1 promotes ATM activity through direct interactions with the MRN complex.
Lee JH, Goodarzi AA, Jeggo PA, Paull TT
The EMBO journal 2010 Feb 3;29(3):574-85
The EMBO journal 2010 Feb 3;29(3):574-85
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Phospho-53BP1 (S25) by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line untreated (-) or exposed (+) to 50 J/m2 UV-C and allowed 1 hour recovery time. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-53BP1 (S25) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3405) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-53BP1 (S25) was detected at approximately 450 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase ( lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
