PA5-34675

antibody from Invitrogen Antibodies

Targeting: TP53BP1 53BP1, p202, TDRD30

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34675

Provider

Invitrogen Antibodies

Product name

53BP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, HeLa, Neuro 2A, C8D30, NIH-3T3.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of 53BP1 using A) 30 µg 293T whole cell lysate and B) 30 whole cell lysate of human 53BP1-transfected 293T cells. Samples were loaded onto a 5% SDS-PAGE gel and probed with a 53BP1 polyclonal antibody (Product # PA5-34675) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

53BP1 Polyclonal Antibody detects TP53BP1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg HeLa whole cell lysate/extract.5 % SDS-PAGE.53BP1 Polyclonal Antibody (Product # PA5-34675) dilution: 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of 53BP1 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a 53BP1 Polyclonal Antibody (Product # PA5-34675) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

53BP1 Polyclonal Antibody detects TP53BP1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg HeLa whole cell lysate/extract.5 % SDS-PAGE.53BP1 Polyclonal Antibody (Product # PA5-34675) dilution: 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of 53BP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: 53BP1 Polyclonal Antibody (Product # PA5-34675) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of 53BP1 was performed in paraffin-embedded human breast carcinoma tissue using 53BP1 Polyclonal Antibody (Product # PA5-34675) at a dilution of 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.