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- Antibody Data
- Antigen structure
- References [93]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB100-305 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-305, RRID:AB_10001695
- Product name
- Rabbit Polyclonal 53BP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Temporal transcriptome analysis of neuronal commitment reveals the preeminent role of the divergent lncRNA biotype and a critical candidate gene during differentiation.
H3K36 dimethylation by MMSET promotes classical non-homologous end-joining at unprotected telomeres.
BRCA1 Mutational Complementation Induces Synthetic Viability.
Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication Is Independent of Anaphase-Promoting Complex Activity.
Extended in vitro culture of primary human mesenchymal stem cells downregulates Brca1-related genes and impairs DNA double-strand break recognition.
53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination.
BubR1 allelic effects drive phenotypic heterogeneity in mosaic-variegated aneuploidy progeria syndrome.
Physiological Tolerance to ssDNA Enables Strand Uncoupling during DNA Replication.
CTCF cooperates with CtIP to drive homologous recombination repair of double-strand breaks.
Inhibition of CDK4/CDK6 Enhances Radiosensitivity of HPV Negative Head and Neck Squamous Cell Carcinomas.
BRCA1 Haploinsufficiency Is Masked by RNF168-Mediated Chromatin Ubiquitylation.
Length-independent telomere damage drives post-mitotic cardiomyocyte senescence.
Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability.
Muscle Stem Cells Give Rise to Rhabdomyosarcomas in a Severe Mouse Model of Duchenne Muscular Dystrophy.
ROS-Induced Activation of DNA Damage Responses Drives Senescence-Like State in Postmitotic Cochlear Cells: Implication for Hearing Preservation.
53BP1 nuclear bodies enforce replication timing at under-replicated DNA to limit heritable DNA damage.
A functional ex vivo assay to detect PARP1-EJ repair and radiosensitization by PARP-inhibitor in prostate cancer.
Comprehensive Mapping of Histone Modifications at DNA Double-Strand Breaks Deciphers Repair Pathway Chromatin Signatures.
PTEN deletion in luminal cells of mature prostate induces replication stress and senescence in vivo.
Histone H3K27 methylation modulates the dynamics of FANCD2 on chromatin to facilitate NHEJ and genome stability.
A Novel Mechanism of γ-Irradiation-Induced IL-6 Production Mediated by P2Y11 Receptor in Epidermal Keratinocytes.
Induction of a Senescence-Like Phenotype in Cultured Human Fetal Microglia During HIV-1 Infection.
The telomeric DNA damage response occurs in the absence of chromatin decompaction.
Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System.
DNA Damage Detection by 53BP1: Relationship to Species Longevity.
Reversible p53 inhibition prevents cisplatin ototoxicity without blocking chemotherapeutic efficacy.
Chromatin organization revealed by nanostructure of irradiation induced γH2AX, 53BP1 and Rad51 foci.
Depletion of Histone Demethylase Jarid1A Resulting in Histone Hyperacetylation and Radiation Sensitivity Does Not Affect DNA Double-Strand Break Repair.
Exosomes Derived from Squamous Head and Neck Cancer Promote Cell Survival after Ionizing Radiation.
Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1.
Inhibition of DNA damage repair by the CDK4/6 inhibitor palbociclib delays irradiated intracranial atypical teratoid rhabdoid tumor and glioblastoma xenograft regrowth.
Functional crosstalk between DNA damage response proteins 53BP1 and BRCA1 regulates double strand break repair choice.
Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes.
XRN2 Links Transcription Termination to DNA Damage and Replication Stress.
TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres.
A novel homozygous splicing mutation of CASC5 causes primary microcephaly in a large Pakistani family.
DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions.
The histone variant H2A.X is a regulator of the epithelial-mesenchymal transition.
53BP1 fosters fidelity of homology-directed DNA repair.
Nanoscopic exclusion between Rad51 and 53BP1 after ion irradiation in human HeLa cells.
MAD2L2 controls DNA repair at telomeres and DNA breaks by inhibiting 5' end resection.
The topoisomerase II catalytic inhibitor ICRF-193 preferentially targets telomeres that are capped by TRF2.
PRKDC mutations associated with immunodeficiency, granuloma, and autoimmune regulator-dependent autoimmunity.
Co-treatment with BEZ235 Enhances Sensitivity of BRCA1-negative Breast Cancer Cells to Olaparib.
DNA damage induces nuclear actin filament assembly by Formin -2 and Spire-½ that promotes efficient DNA repair. [corrected].
Exit from dormancy provokes DNA-damage-induced attrition in haematopoietic stem cells.
ATRX dysfunction induces replication defects in primary mouse cells.
miR-214-mediated downregulation of RNF8 induces chromosomal instability in ovarian cancer cells.
SET8 methyltransferase activity during the DNA double-strand break response is required for recruitment of 53BP1.
DNA double strand break responses and chromatin alterations within the aging cell.
The tumor suppressor PML specifically accumulates at RPA/Rad51-containing DNA damage repair foci but is nonessential for DNA damage-induced fibroblast senescence.
Senescence induced by RECQL4 dysfunction contributes to Rothmund-Thomson syndrome features in mice.
Chronic inflammation induces telomere dysfunction and accelerates ageing in mice.
Fine-tuning of DNA damage-dependent ubiquitination by OTUB2 supports the DNA repair pathway choice.
Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage.
Tumor cell death mediated by peptides that recognize branched intermediates of DNA replication and repair.
Inactivation of HNSCC cells by 90Y-labeled cetuximab strictly depends on the number of induced DNA double-strand breaks.
Acetylation limits 53BP1 association with damaged chromatin to promote homologous recombination.
TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
53BP1 regulates DSB repair using Rif1 to control 5' end resection.
Acacetin and chrysin, two polyphenolic compounds, alleviate telomeric position effect in human cells.
Concurrent V(D)J recombination and DNA end instability increase interchromosomal trans-rearrangements in ATM-deficient thymocytes.
Loss of PIDD limits NF-κB activation and cytokine production but not cell survival or transformation after DNA damage.
Necrosis-like death can engage multiple pro-apoptotic Bcl-2 protein family members.
Cisplatin resistance induced in germ cell tumour cells is due to reduced susceptibility towards cell death but not to altered DNA damage induction or repair.
Shelterin dysfunction and p16(INK4a)-mediated growth inhibition in HIV-1-specific CD8 T cells.
The DNA translocase activity of FANCM protects stalled replication forks.
Postmitotic neurons develop a p21-dependent senescence-like phenotype driven by a DNA damage response.
BRCA1-associated exclusion of 53BP1 from DNA damage sites underlies temporal control of DNA repair.
The ATPase activity of MLH1 is required to orchestrate DNA double-strand breaks and end processing during class switch recombination.
First do no harm.
Nature 2011 Jan 13;469(7329):132
DNA-SCARS: distinct nuclear structures that sustain damage-induced senescence growth arrest and inflammatory cytokine secretion.
Development and application of an enzymatic and cell flotation treatment for the recovery of viable microbial cells from environmental matrices such as anaerobic sludge.
WRN helicase regulates the ATR-CHK1-induced S-phase checkpoint pathway in response to topoisomerase-I-DNA covalent complexes.
Telomeric damage in early stage of chronic lymphocytic leukemia correlates with shelterin dysregulation.
DNA-damage response and repair activities at uncapped telomeres depend on RNF8.
p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype.
The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination.
Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1.
MERIT40 controls BRCA1-Rap80 complex integrity and recruitment to DNA double-strand breaks.
Functional dissection of human and mouse POT1 proteins.
Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways.
The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage.
A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells.
Transcription-coupled DNA double-strand breaks are mediated via the nucleotide excision repair and the Mre11-Rad50-Nbs1 complex.
Competition effect in DNA damage response.
Quantitative analysis of DNA-damage response factors after sequential ion microirradiation.
Excess MCM proteins protect human cells from replicative stress by licensing backup origins of replication.
Impaired DNA double-strand break repair contributes to chemoresistance in HIF-1 alpha-deficient mouse embryonic fibroblasts.
HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies.
Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure.
Quantitative analysis of radiation-induced DNA break repair in a cultured oral mucosal model.
Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint.
Prajapati B, Fatima M, Fatma M, Maddhesiya P, Arora H, Naskar T, Devasenapathy S, Seth P, Sinha S
Cell death discovery 2020;6:28
Cell death discovery 2020;6:28
H3K36 dimethylation by MMSET promotes classical non-homologous end-joining at unprotected telomeres.
de Krijger I, van der Torre J, Peuscher MH, Eder M, Jacobs JJL
Oncogene 2020 Jun;39(25):4814-4827
Oncogene 2020 Jun;39(25):4814-4827
BRCA1 Mutational Complementation Induces Synthetic Viability.
Nacson J, Di Marcantonio D, Wang Y, Bernhardy AJ, Clausen E, Hua X, Cai KQ, Martinez E, Feng W, Callén E, Wu W, Gupta GP, Testa JR, Nussenzweig A, Sykes SM, Johnson N
Molecular cell 2020 Jun 4;78(5):951-959.e6
Molecular cell 2020 Jun 4;78(5):951-959.e6
Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication Is Independent of Anaphase-Promoting Complex Activity.
Elbasani E, Gramolelli S, Günther T, Gabaev I, Grundhoff A, Ojala PM
Journal of virology 2020 Jun 16;94(13)
Journal of virology 2020 Jun 16;94(13)
Extended in vitro culture of primary human mesenchymal stem cells downregulates Brca1-related genes and impairs DNA double-strand break recognition.
Bao X, Wang J, Zhou G, Aszodi A, Schönitzer V, Scherthan H, Atkinson MJ, Rosemann M
FEBS open bio 2020 Jul;10(7):1238-1250
FEBS open bio 2020 Jul;10(7):1238-1250
53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination.
Callen E, Zong D, Wu W, Wong N, Stanlie A, Ishikawa M, Pavani R, Dumitrache LC, Byrum AK, Mendez-Dorantes C, Martinez P, Canela A, Maman Y, Day A, Kruhlak MJ, Blasco MA, Stark JM, Mosammaparast N, McKinnon PJ, Nussenzweig A
Molecular cell 2020 Jan 2;77(1):26-38.e7
Molecular cell 2020 Jan 2;77(1):26-38.e7
BubR1 allelic effects drive phenotypic heterogeneity in mosaic-variegated aneuploidy progeria syndrome.
Sieben CJ, Jeganathan KB, Nelson GG, Sturmlechner I, Zhang C, van Deursen WH, Bakker B, Foijer F, Li H, Baker DJ, van Deursen JM
The Journal of clinical investigation 2020 Jan 2;130(1):171-188
The Journal of clinical investigation 2020 Jan 2;130(1):171-188
Physiological Tolerance to ssDNA Enables Strand Uncoupling during DNA Replication.
Ercilla A, Benada J, Amitash S, Zonderland G, Baldi G, Somyajit K, Ochs F, Costanzo V, Lukas J, Toledo L
Cell reports 2020 Feb 18;30(7):2416-2429.e7
Cell reports 2020 Feb 18;30(7):2416-2429.e7
CTCF cooperates with CtIP to drive homologous recombination repair of double-strand breaks.
Hwang SY, Kang MA, Baik CJ, Lee Y, Hang NT, Kim BG, Han JS, Jeong JH, Park D, Myung K, Lee JS
Nucleic acids research 2019 Sep 26;47(17):9160-9179
Nucleic acids research 2019 Sep 26;47(17):9160-9179
Inhibition of CDK4/CDK6 Enhances Radiosensitivity of HPV Negative Head and Neck Squamous Cell Carcinomas.
Göttgens EL, Bussink J, Leszczynska KB, Peters H, Span PN, Hammond EM
International journal of radiation oncology, biology, physics 2019 Nov 1;105(3):548-558
International journal of radiation oncology, biology, physics 2019 Nov 1;105(3):548-558
BRCA1 Haploinsufficiency Is Masked by RNF168-Mediated Chromatin Ubiquitylation.
Zong D, Adam S, Wang Y, Sasanuma H, Callén E, Murga M, Day A, Kruhlak MJ, Wong N, Munro M, Ray Chaudhuri A, Karim B, Xia B, Takeda S, Johnson N, Durocher D, Nussenzweig A
Molecular cell 2019 Mar 21;73(6):1267-1281.e7
Molecular cell 2019 Mar 21;73(6):1267-1281.e7
Length-independent telomere damage drives post-mitotic cardiomyocyte senescence.
Anderson R, Lagnado A, Maggiorani D, Walaszczyk A, Dookun E, Chapman J, Birch J, Salmonowicz H, Ogrodnik M, Jurk D, Proctor C, Correia-Melo C, Victorelli S, Fielder E, Berlinguer-Palmini R, Owens A, Greaves LC, Kolsky KL, Parini A, Douin-Echinard V, LeBrasseur NK, Arthur HM, Tual-Chalot S, Schafer MJ, Roos CM, Miller JD, Robertson N, Mann J, Adams PD, Tchkonia T, Kirkland JL, Mialet-Perez J, Richardson GD, Passos JF
The EMBO journal 2019 Mar 1;38(5)
The EMBO journal 2019 Mar 1;38(5)
Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability.
Hladik D, Höfig I, Oestreicher U, Beckers J, Matjanovski M, Bao X, Scherthan H, Atkinson MJ, Rosemann M
Stem cell research & therapy 2019 Jul 29;10(1):218
Stem cell research & therapy 2019 Jul 29;10(1):218
Muscle Stem Cells Give Rise to Rhabdomyosarcomas in a Severe Mouse Model of Duchenne Muscular Dystrophy.
Boscolo Sesillo F, Fox D, Sacco A
Cell reports 2019 Jan 15;26(3):689-701.e6
Cell reports 2019 Jan 15;26(3):689-701.e6
ROS-Induced Activation of DNA Damage Responses Drives Senescence-Like State in Postmitotic Cochlear Cells: Implication for Hearing Preservation.
Benkafadar N, François F, Affortit C, Casas F, Ceccato JC, Menardo J, Venail F, Malfroy-Camine B, Puel JL, Wang J
Molecular neurobiology 2019 Aug;56(8):5950-5969
Molecular neurobiology 2019 Aug;56(8):5950-5969
53BP1 nuclear bodies enforce replication timing at under-replicated DNA to limit heritable DNA damage.
Spies J, Lukas C, Somyajit K, Rask MB, Lukas J, Neelsen KJ
Nature cell biology 2019 Apr;21(4):487-497
Nature cell biology 2019 Apr;21(4):487-497
A functional ex vivo assay to detect PARP1-EJ repair and radiosensitization by PARP-inhibitor in prostate cancer.
Köcher S, Beyer B, Lange T, Nordquist L, Volquardsen J, Burdak-Rothkamm S, Schlomm T, Petersen C, Rothkamm K, Mansour WY
International journal of cancer 2019 Apr 1;144(7):1685-1696
International journal of cancer 2019 Apr 1;144(7):1685-1696
Comprehensive Mapping of Histone Modifications at DNA Double-Strand Breaks Deciphers Repair Pathway Chromatin Signatures.
Clouaire T, Rocher V, Lashgari A, Arnould C, Aguirrebengoa M, Biernacka A, Skrzypczak M, Aymard F, Fongang B, Dojer N, Iacovoni JS, Rowicka M, Ginalski K, Côté J, Legube G
Molecular cell 2018 Oct 18;72(2):250-262.e6
Molecular cell 2018 Oct 18;72(2):250-262.e6
PTEN deletion in luminal cells of mature prostate induces replication stress and senescence in vivo.
Parisotto M, Grelet E, El Bizri R, Dai Y, Terzic J, Eckert D, Gargowitsch L, Bornert JM, Metzger D
The Journal of experimental medicine 2018 Jun 4;215(6):1749-1763
The Journal of experimental medicine 2018 Jun 4;215(6):1749-1763
Histone H3K27 methylation modulates the dynamics of FANCD2 on chromatin to facilitate NHEJ and genome stability.
Zhang Y, Chang JF, Sun J, Chen L, Yang XM, Tang HY, Jing YY, Kang X, He ZM, Wu JY, Wei HM, Wang DL, Xu RG, Zhu RB, Shen Y, Zeng SY, Wang C, Liu KN, Zhang Y, Mao ZY, Jiang CZ, Sun FL
Journal of cell science 2018 Jun 21;131(12)
Journal of cell science 2018 Jun 21;131(12)
A Novel Mechanism of γ-Irradiation-Induced IL-6 Production Mediated by P2Y11 Receptor in Epidermal Keratinocytes.
Ohsaki A, Miyano Y, Tanaka R, Tanuma SI, Kojima S, Tsukimoto M
Biological & pharmaceutical bulletin 2018 Jun 1;41(6):925-936
Biological & pharmaceutical bulletin 2018 Jun 1;41(6):925-936
Induction of a Senescence-Like Phenotype in Cultured Human Fetal Microglia During HIV-1 Infection.
Chen NC, Partridge AT, Tuzer F, Cohen J, Nacarelli T, Navas-Martín S, Sell C, Torres C, Martín-García J
The journals of gerontology. Series A, Biological sciences and medical sciences 2018 Aug 10;73(9):1187-1196
The journals of gerontology. Series A, Biological sciences and medical sciences 2018 Aug 10;73(9):1187-1196
The telomeric DNA damage response occurs in the absence of chromatin decompaction.
Vancevska A, Douglass KM, Pfeiffer V, Manley S, Lingner J
Genes & development 2017 Mar 15;31(6):567-577
Genes & development 2017 Mar 15;31(6):567-577
Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System.
Santaguida S, Richardson A, Iyer DR, M'Saad O, Zasadil L, Knouse KA, Wong YL, Rhind N, Desai A, Amon A
Developmental cell 2017 Jun 19;41(6):638-651.e5
Developmental cell 2017 Jun 19;41(6):638-651.e5
DNA Damage Detection by 53BP1: Relationship to Species Longevity.
Croco E, Marchionni S, Bocchini M, Angeloni C, Stamato T, Stefanelli C, Hrelia S, Sell C, Lorenzini A
The journals of gerontology. Series A, Biological sciences and medical sciences 2017 Jun 1;72(6):763-770
The journals of gerontology. Series A, Biological sciences and medical sciences 2017 Jun 1;72(6):763-770
Reversible p53 inhibition prevents cisplatin ototoxicity without blocking chemotherapeutic efficacy.
Benkafadar N, Menardo J, Bourien J, Nouvian R, François F, Decaudin D, Maiorano D, Puel JL, Wang J
EMBO molecular medicine 2017 Jan;9(1):7-26
EMBO molecular medicine 2017 Jan;9(1):7-26
Chromatin organization revealed by nanostructure of irradiation induced γH2AX, 53BP1 and Rad51 foci.
Reindl J, Girst S, Walsh DW, Greubel C, Schwarz B, Siebenwirth C, Drexler GA, Friedl AA, Dollinger G
Scientific reports 2017 Jan 17;7:40616
Scientific reports 2017 Jan 17;7:40616
Depletion of Histone Demethylase Jarid1A Resulting in Histone Hyperacetylation and Radiation Sensitivity Does Not Affect DNA Double-Strand Break Repair.
Penterling C, Drexler GA, Böhland C, Stamp R, Wilke C, Braselmann H, Caldwell RB, Reindl J, Girst S, Greubel C, Siebenwirth C, Mansour WY, Borgmann K, Dollinger G, Unger K, Friedl AA
PloS one 2016;11(6):e0156599
PloS one 2016;11(6):e0156599
Exosomes Derived from Squamous Head and Neck Cancer Promote Cell Survival after Ionizing Radiation.
Mutschelknaus L, Peters C, Winkler K, Yentrapalli R, Heider T, Atkinson MJ, Moertl S
PloS one 2016;11(3):e0152213
PloS one 2016;11(3):e0152213
Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1.
Bakr A, Köcher S, Volquardsen J, Petersen C, Borgmann K, Dikomey E, Rothkamm K, Mansour WY
Oncotarget 2016 Sep 6;7(36):57679-57693
Oncotarget 2016 Sep 6;7(36):57679-57693
Inhibition of DNA damage repair by the CDK4/6 inhibitor palbociclib delays irradiated intracranial atypical teratoid rhabdoid tumor and glioblastoma xenograft regrowth.
Hashizume R, Zhang A, Mueller S, Prados MD, Lulla RR, Goldman S, Saratsis AM, Mazar AP, Stegh AH, Cheng SY, Horbinski C, Haas-Kogan DA, Sarkaria JN, Waldman T, James CD
Neuro-oncology 2016 Nov;18(11):1519-1528
Neuro-oncology 2016 Nov;18(11):1519-1528
Functional crosstalk between DNA damage response proteins 53BP1 and BRCA1 regulates double strand break repair choice.
Bakr A, Köcher S, Volquardsen J, Reimer R, Borgmann K, Dikomey E, Rothkamm K, Mansour WY
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2016 May;119(2):276-81
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2016 May;119(2):276-81
Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes.
van Ree JH, Nam HJ, Jeganathan KB, Kanakkanthara A, van Deursen JM
Nature cell biology 2016 Jul;18(7):814-21
Nature cell biology 2016 Jul;18(7):814-21
XRN2 Links Transcription Termination to DNA Damage and Replication Stress.
Morales JC, Richard P, Patidar PL, Motea EA, Dang TT, Manley JL, Boothman DA
PLoS genetics 2016 Jul;12(7):e1006107
PLoS genetics 2016 Jul;12(7):e1006107
TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres.
Kibe T, Zimmermann M, de Lange T
Molecular cell 2016 Jan 21;61(2):236-46
Molecular cell 2016 Jan 21;61(2):236-46
A novel homozygous splicing mutation of CASC5 causes primary microcephaly in a large Pakistani family.
Szczepanski S, Hussain MS, Sur I, Altmüller J, Thiele H, Abdullah U, Waseem SS, Moawia A, Nürnberg G, Noegel AA, Baig SM, Nürnberg P
Human genetics 2016 Feb;135(2):157-70
Human genetics 2016 Feb;135(2):157-70
DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions.
Cristini A, Park JH, Capranico G, Legube G, Favre G, Sordet O
Nucleic acids research 2016 Feb 18;44(3):1161-78
Nucleic acids research 2016 Feb 18;44(3):1161-78
The histone variant H2A.X is a regulator of the epithelial-mesenchymal transition.
Weyemi U, Redon CE, Choudhuri R, Aziz T, Maeda D, Boufraqech M, Parekh PR, Sethi TK, Kasoji M, Abrams N, Merchant A, Rajapakse VN, Bonner WM
Nature communications 2016 Feb 15;7:10711
Nature communications 2016 Feb 15;7:10711
53BP1 fosters fidelity of homology-directed DNA repair.
Ochs F, Somyajit K, Altmeyer M, Rask MB, Lukas J, Lukas C
Nature structural & molecular biology 2016 Aug;23(8):714-21
Nature structural & molecular biology 2016 Aug;23(8):714-21
Nanoscopic exclusion between Rad51 and 53BP1 after ion irradiation in human HeLa cells.
Reindl J, Drexler GA, Girst S, Greubel C, Siebenwirth C, Drexler SE, Dollinger G, Friedl AA
Physical biology 2015 Nov 23;12(6):066005
Physical biology 2015 Nov 23;12(6):066005
MAD2L2 controls DNA repair at telomeres and DNA breaks by inhibiting 5' end resection.
Boersma V, Moatti N, Segura-Bayona S, Peuscher MH, van der Torre J, Wevers BA, Orthwein A, Durocher D, Jacobs JJL
Nature 2015 May 28;521(7553):537-540
Nature 2015 May 28;521(7553):537-540
The topoisomerase II catalytic inhibitor ICRF-193 preferentially targets telomeres that are capped by TRF2.
Chen L, Zhu X, Zou Y, Xing J, Gilson E, Lu Y, Ye J
American journal of physiology. Cell physiology 2015 Mar 1;308(5):C372-7
American journal of physiology. Cell physiology 2015 Mar 1;308(5):C372-7
PRKDC mutations associated with immunodeficiency, granuloma, and autoimmune regulator-dependent autoimmunity.
Mathieu AL, Verronese E, Rice GI, Fouyssac F, Bertrand Y, Picard C, Chansel M, Walter JE, Notarangelo LD, Butte MJ, Nadeau KC, Csomos K, Chen DJ, Chen K, Delgado A, Rigal C, Bardin C, Schuetz C, Moshous D, Reumaux H, Plenat F, Phan A, Zabot MT, Balme B, Viel S, Bienvenu J, Cochat P, van der Burg M, Caux C, Kemp EH, Rouvet I, Malcus C, Méritet JF, Lim A, Crow YJ, Fabien N, Ménétrier-Caux C, De Villartay JP, Walzer T, Belot A
The Journal of allergy and clinical immunology 2015 Jun;135(6):1578-88.e5
The Journal of allergy and clinical immunology 2015 Jun;135(6):1578-88.e5
Co-treatment with BEZ235 Enhances Sensitivity of BRCA1-negative Breast Cancer Cells to Olaparib.
Yi YW, Park JS, Kwak SJ, Seong YS
Anticancer research 2015 Jul;35(7):3829-38
Anticancer research 2015 Jul;35(7):3829-38
DNA damage induces nuclear actin filament assembly by Formin -2 and Spire-½ that promotes efficient DNA repair. [corrected].
Belin BJ, Lee T, Mullins RD
eLife 2015 Aug 19;4:e07735
eLife 2015 Aug 19;4:e07735
Exit from dormancy provokes DNA-damage-induced attrition in haematopoietic stem cells.
Walter D, Lier A, Geiselhart A, Thalheimer FB, Huntscha S, Sobotta MC, Moehrle B, Brocks D, Bayindir I, Kaschutnig P, Muedder K, Klein C, Jauch A, Schroeder T, Geiger H, Dick TP, Holland-Letz T, Schmezer P, Lane SW, Rieger MA, Essers MA, Williams DA, Trumpp A, Milsom MD
Nature 2015 Apr 23;520(7548):549-52
Nature 2015 Apr 23;520(7548):549-52
ATRX dysfunction induces replication defects in primary mouse cells.
Clynes D, Jelinska C, Xella B, Ayyub H, Taylor S, Mitson M, Bachrati CZ, Higgs DR, Gibbons RJ
PloS one 2014;9(3):e92915
PloS one 2014;9(3):e92915
miR-214-mediated downregulation of RNF8 induces chromosomal instability in ovarian cancer cells.
Wang Z, Yin H, Zhang Y, Feng Y, Yan Z, Jiang X, Bukhari I, Iqbal F, Cooke HJ, Shi Q
Cell cycle (Georgetown, Tex.) 2014;13(22):3519-28
Cell cycle (Georgetown, Tex.) 2014;13(22):3519-28
SET8 methyltransferase activity during the DNA double-strand break response is required for recruitment of 53BP1.
Dulev S, Tkach J, Lin S, Batada NN
EMBO reports 2014 Nov;15(11):1163-74
EMBO reports 2014 Nov;15(11):1163-74
DNA double strand break responses and chromatin alterations within the aging cell.
Klement K, Goodarzi AA
Experimental cell research 2014 Nov 15;329(1):42-52
Experimental cell research 2014 Nov 15;329(1):42-52
The tumor suppressor PML specifically accumulates at RPA/Rad51-containing DNA damage repair foci but is nonessential for DNA damage-induced fibroblast senescence.
Münch S, Weidtkamp-Peters S, Klement K, Grigaravicius P, Monajembashi S, Salomoni P, Pandolfi PP, Weißhart K, Hemmerich P
Molecular and cellular biology 2014 May;34(10):1733-46
Molecular and cellular biology 2014 May;34(10):1733-46
Senescence induced by RECQL4 dysfunction contributes to Rothmund-Thomson syndrome features in mice.
Lu H, Fang EF, Sykora P, Kulikowicz T, Zhang Y, Becker KG, Croteau DL, Bohr VA
Cell death & disease 2014 May 15;5(5):e1226
Cell death & disease 2014 May 15;5(5):e1226
Chronic inflammation induces telomere dysfunction and accelerates ageing in mice.
Jurk D, Wilson C, Passos JF, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R, Hewitt G, Pender SL, Fullard N, Nelson G, Mann J, van de Sluis B, Mann DA, von Zglinicki T
Nature communications 2014 Jun 24;2:4172
Nature communications 2014 Jun 24;2:4172
Fine-tuning of DNA damage-dependent ubiquitination by OTUB2 supports the DNA repair pathway choice.
Kato K, Nakajima K, Ui A, Muto-Terao Y, Ogiwara H, Nakada S
Molecular cell 2014 Feb 20;53(4):617-30
Molecular cell 2014 Feb 20;53(4):617-30
Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage.
Cousin W, Ho ML, Desai R, Tham A, Chen RY, Kung S, Elabd C, Conboy IM
PloS one 2013;8(5):e63528
PloS one 2013;8(5):e63528
Tumor cell death mediated by peptides that recognize branched intermediates of DNA replication and repair.
Dey M, Patra S, Su LY, Segall AM
PloS one 2013;8(11):e78751
PloS one 2013;8(11):e78751
Inactivation of HNSCC cells by 90Y-labeled cetuximab strictly depends on the number of induced DNA double-strand breaks.
Saker J, Kriegs M, Zenker M, Heldt JM, Eke I, Pietzsch HJ, Grénman R, Cordes N, Petersen C, Baumann M, Steinbach J, Dikomey E, Kasten-Pisula U
Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2013 Mar;54(3):416-23
Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2013 Mar;54(3):416-23
Acetylation limits 53BP1 association with damaged chromatin to promote homologous recombination.
Tang J, Cho NW, Cui G, Manion EM, Shanbhag NM, Botuyan MV, Mer G, Greenberg RA
Nature structural & molecular biology 2013 Mar;20(3):317-25
Nature structural & molecular biology 2013 Mar;20(3):317-25
TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
Biroccio A, Cherfils-Vicini J, Augereau A, Pinte S, Bauwens S, Ye J, Simonet T, Horard B, Jamet K, Cervera L, Mendez-Bermudez A, Poncet D, Grataroli R, de Rodenbeeke CT, Salvati E, Rizzo A, Zizza P, Ricoul M, Cognet C, Kuilman T, Duret H, Lépinasse F, Marvel J, Verhoeyen E, Cosset FL, Peeper D, Smyth MJ, Londoño-Vallejo A, Sabatier L, Picco V, Pages G, Scoazec JY, Stoppacciaro A, Leonetti C, Vivier E, Gilson E
Nature cell biology 2013 Jul;15(7):818-28
Nature cell biology 2013 Jul;15(7):818-28
53BP1 regulates DSB repair using Rif1 to control 5' end resection.
Zimmermann M, Lottersberger F, Buonomo SB, Sfeir A, de Lange T
Science (New York, N.Y.) 2013 Feb 8;339(6120):700-4
Science (New York, N.Y.) 2013 Feb 8;339(6120):700-4
Acacetin and chrysin, two polyphenolic compounds, alleviate telomeric position effect in human cells.
Boussouar A, Barette C, Nadon R, Saint-Léger A, Broucqsault N, Ottaviani A, Firozhoussen A, Lu Y, Lafanechère L, Gilson E, Magdinier F, Ye J
Molecular therapy. Nucleic acids 2013 Aug 20;2:e116
Molecular therapy. Nucleic acids 2013 Aug 20;2:e116
Concurrent V(D)J recombination and DNA end instability increase interchromosomal trans-rearrangements in ATM-deficient thymocytes.
Bowen S, Wangsa D, Ried T, Livak F, Hodes RJ
Nucleic acids research 2013 Apr;41(8):4535-48
Nucleic acids research 2013 Apr;41(8):4535-48
Loss of PIDD limits NF-κB activation and cytokine production but not cell survival or transformation after DNA damage.
Bock FJ, Krumschnabel G, Manzl C, Peintner L, Tanzer MC, Hermann-Kleiter N, Baier G, Llacuna L, Yelamos J, Villunger A
Cell death and differentiation 2013 Apr;20(4):546-57
Cell death and differentiation 2013 Apr;20(4):546-57
Necrosis-like death can engage multiple pro-apoptotic Bcl-2 protein family members.
Tischner D, Manzl C, Soratroi C, Villunger A, Krumschnabel G
Apoptosis : an international journal on programmed cell death 2012 Nov;17(11):1197-1209
Apoptosis : an international journal on programmed cell death 2012 Nov;17(11):1197-1209
Cisplatin resistance induced in germ cell tumour cells is due to reduced susceptibility towards cell death but not to altered DNA damage induction or repair.
Fenske AE, Glaesener S, Bokemeyer C, Thomale J, Dahm-Daphi J, Honecker F, Dartsch DC
Cancer letters 2012 Nov 28;324(2):171-8
Cancer letters 2012 Nov 28;324(2):171-8
Shelterin dysfunction and p16(INK4a)-mediated growth inhibition in HIV-1-specific CD8 T cells.
Lichterfeld M, Cung T, Seiss K, Rosenberg ES, Pereyra F, Yu XG
Journal of virology 2012 May;86(10):5533-40
Journal of virology 2012 May;86(10):5533-40
The DNA translocase activity of FANCM protects stalled replication forks.
Blackford AN, Schwab RA, Nieminuszczy J, Deans AJ, West SC, Niedzwiedz W
Human molecular genetics 2012 May 1;21(9):2005-16
Human molecular genetics 2012 May 1;21(9):2005-16
Postmitotic neurons develop a p21-dependent senescence-like phenotype driven by a DNA damage response.
Jurk D, Wang C, Miwa S, Maddick M, Korolchuk V, Tsolou A, Gonos ES, Thrasivoulou C, Saffrey MJ, Cameron K, von Zglinicki T
Aging cell 2012 Dec;11(6):996-1004
Aging cell 2012 Dec;11(6):996-1004
BRCA1-associated exclusion of 53BP1 from DNA damage sites underlies temporal control of DNA repair.
Chapman JR, Sossick AJ, Boulton SJ, Jackson SP
Journal of cell science 2012 Aug 1;125(Pt 15):3529-34
Journal of cell science 2012 Aug 1;125(Pt 15):3529-34
The ATPase activity of MLH1 is required to orchestrate DNA double-strand breaks and end processing during class switch recombination.
Chahwan R, van Oers JM, Avdievich E, Zhao C, Edelmann W, Scharff MD, Roa S
The Journal of experimental medicine 2012 Apr 9;209(4):671-8
The Journal of experimental medicine 2012 Apr 9;209(4):671-8
First do no harm.
Nature 2011 Jan 13;469(7329):132
DNA-SCARS: distinct nuclear structures that sustain damage-induced senescence growth arrest and inflammatory cytokine secretion.
Rodier F, Muñoz DP, Teachenor R, Chu V, Le O, Bhaumik D, Coppé JP, Campeau E, Beauséjour CM, Kim SH, Davalos AR, Campisi J
Journal of cell science 2011 Jan 1;124(Pt 1):68-81
Journal of cell science 2011 Jan 1;124(Pt 1):68-81
Development and application of an enzymatic and cell flotation treatment for the recovery of viable microbial cells from environmental matrices such as anaerobic sludge.
Braun F, Hamelin J, Gévaudan G, Patureau D
Applied and environmental microbiology 2011 Dec;77(24):8487-93
Applied and environmental microbiology 2011 Dec;77(24):8487-93
WRN helicase regulates the ATR-CHK1-induced S-phase checkpoint pathway in response to topoisomerase-I-DNA covalent complexes.
Patro BS, Frøhlich R, Bohr VA, Stevnsner T
Journal of cell science 2011 Dec 1;124(Pt 23):3967-79
Journal of cell science 2011 Dec 1;124(Pt 23):3967-79
Telomeric damage in early stage of chronic lymphocytic leukemia correlates with shelterin dysregulation.
Augereau A, T'kint de Roodenbeke C, Simonet T, Bauwens S, Horard B, Callanan M, Leroux D, Jallades L, Salles G, Gilson E, Poncet D
Blood 2011 Aug 4;118(5):1316-22
Blood 2011 Aug 4;118(5):1316-22
DNA-damage response and repair activities at uncapped telomeres depend on RNF8.
Peuscher MH, Jacobs JJ
Nature cell biology 2011 Aug 21;13(9):1139-45
Nature cell biology 2011 Aug 21;13(9):1139-45
p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype.
Freund A, Patil CK, Campisi J
The EMBO journal 2011 Apr 20;30(8):1536-48
The EMBO journal 2011 Apr 20;30(8):1536-48
The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination.
Ramachandran S, Chahwan R, Nepal RM, Frieder D, Panier S, Roa S, Zaheen A, Durocher D, Scharff MD, Martin A
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 12;107(2):809-14
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 12;107(2):809-14
Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1.
Nakada S, Tai I, Panier S, Al-Hakim A, Iemura S, Juang YC, O'Donnell L, Kumakubo A, Munro M, Sicheri F, Gingras AC, Natsume T, Suda T, Durocher D
Nature 2010 Aug 19;466(7309):941-6
Nature 2010 Aug 19;466(7309):941-6
MERIT40 controls BRCA1-Rap80 complex integrity and recruitment to DNA double-strand breaks.
Shao G, Patterson-Fortin J, Messick TE, Feng D, Shanbhag N, Wang Y, Greenberg RA
Genes & development 2009 Mar 15;23(6):740-54
Genes & development 2009 Mar 15;23(6):740-54
Functional dissection of human and mouse POT1 proteins.
Palm W, Hockemeyer D, Kibe T, de Lange T
Molecular and cellular biology 2009 Jan;29(2):471-82
Molecular and cellular biology 2009 Jan;29(2):471-82
Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways.
Solier S, Sordet O, Kohn KW, Pommier Y
Molecular and cellular biology 2009 Jan;29(1):68-82
Molecular and cellular biology 2009 Jan;29(1):68-82
The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage.
Stewart GS, Panier S, Townsend K, Al-Hakim AK, Kolas NK, Miller ES, Nakada S, Ylanko J, Olivarius S, Mendez M, Oldreive C, Wildenhain J, Tagliaferro A, Pelletier L, Taubenheim N, Durandy A, Byrd PJ, Stankovic T, Taylor AM, Durocher D
Cell 2009 Feb 6;136(3):420-34
Cell 2009 Feb 6;136(3):420-34
A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells.
Rieske P, Augelli BJ, Stawski R, Gaughan J, Azizi SA, Krynska B
Experimental cell research 2009 Feb 1;315(3):462-73
Experimental cell research 2009 Feb 1;315(3):462-73
Transcription-coupled DNA double-strand breaks are mediated via the nucleotide excision repair and the Mre11-Rad50-Nbs1 complex.
Guirouilh-Barbat J, Redon C, Pommier Y
Molecular biology of the cell 2008 Sep;19(9):3969-81
Molecular biology of the cell 2008 Sep;19(9):3969-81
Competition effect in DNA damage response.
Greubel C, Hable V, Drexler GA, Hauptner A, Dietzel S, Strickfaden H, Baur I, Krücken R, Cremer T, Dollinger G, Friedl AA
Radiation and environmental biophysics 2008 Nov;47(4):423-9
Radiation and environmental biophysics 2008 Nov;47(4):423-9
Quantitative analysis of DNA-damage response factors after sequential ion microirradiation.
Greubel C, Hable V, Drexler GA, Hauptner A, Dietzel S, Strickfaden H, Baur I, Krücken R, Cremer T, Friedl AA, Dollinger G
Radiation and environmental biophysics 2008 Nov;47(4):415-22
Radiation and environmental biophysics 2008 Nov;47(4):415-22
Excess MCM proteins protect human cells from replicative stress by licensing backup origins of replication.
Ibarra A, Schwob E, Méndez J
Proceedings of the National Academy of Sciences of the United States of America 2008 Jul 1;105(26):8956-61
Proceedings of the National Academy of Sciences of the United States of America 2008 Jul 1;105(26):8956-61
Impaired DNA double-strand break repair contributes to chemoresistance in HIF-1 alpha-deficient mouse embryonic fibroblasts.
Wirthner R, Wrann S, Balamurugan K, Wenger RH, Stiehl DP
Carcinogenesis 2008 Dec;29(12):2306-16
Carcinogenesis 2008 Dec;29(12):2306-16
HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies.
Spardy N, Duensing A, Hoskins EE, Wells SI, Duensing S
Cancer research 2008 Dec 1;68(23):9954-63
Cancer research 2008 Dec 1;68(23):9954-63
Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure.
Rothkamm K, Balroop S, Shekhdar J, Fernie P, Goh V
Radiology 2007 Jan;242(1):244-51
Radiology 2007 Jan;242(1):244-51
Quantitative analysis of radiation-induced DNA break repair in a cultured oral mucosal model.
Rakhorst HA, Tra WM, Posthumus-Van Sluijs ST, Hovius SE, Levendag PC, Kanaar R, Hofer SO
Tissue engineering 2006 Dec;12(12):3395-403
Tissue engineering 2006 Dec;12(12):3395-403
Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint.
Silverman J, Takai H, Buonomo SB, Eisenhaber F, de Lange T
Genes & development 2004 Sep 1;18(17):2108-19
Genes & development 2004 Sep 1;18(17):2108-19
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 53BP1 Antibody [NB100-305] - Western blot of whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8 percent trisacetate gel. A band appears at approximately the theoretical molecular weight of 214 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: 53BP1 Antibody [NB100-305] - Immunohistochemical staining of placental villi with 53BP1 Antibody (Catalog #NB100-305) at 40X magnification.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: 53BP1 Antibody [NB100-305] - An intracellular stain was performed on HeLa cells with 53BP1 Antibody (Catalog #NB100-305AF488) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
