Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [3]
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Validation data
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- Product number
- NBP2-27167 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal TLR3 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLR3 Antibody [NBP2-27167] - Mouse testis tissue probed with 5 ug/ml TLR3 antibody in the absence (1) and presence (2) of immunizing peptide. A goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TLR3 Antibody [NBP2-27167] - Mouse testis tissue probed with TLR3 antibody at 5 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR3 Antibody [NBP2-27167] - Intracellular analysis of TLR3 in mouse splenocytes using TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR3 Antibody [NBP2-27167] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-27167. Staining of TLR3 in human monocytes using 2 ug of this antibody. Green represents isotype control; red represents TLR3 antibody. TLR intracellular flow kit was used for this test.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR3 Antibody [NBP2-27167] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27167. Staining of TLR3 in human monocytes using 2 ug of this antibody. Green represents isotype control antibody; red represents TLR3 antibody. TLR intracellular flow kit was used for this test.