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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [3]
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- Product number
- MA1-25649 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLR3 Monoclonal Antibody (40C1285)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA1-25649 detects TLR3 from human samples.
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 40C1285
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TLR3 was performed by loading (A) 293 cells, (B) 293 cells overexpressing human TLR3, (C) human intestine, (D) placenta, (E) heart and (F) ovary tissue lysates. Proteins were transferred to a membrane and probed with a TLR3 Monoclonal Antibody (40C1285) (Product # MA1-25649) at a dilution of 3 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TLR3 in mouse colon tissue using TLR3 Monoclonal Antibody (40C1285) (Product # MA1-25649) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TLR3 in mouse intestine tissue using TLR3 Monoclonal Antibody (40C1285) (Product # MA1-25649) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of IBA1 was performed in human B cells using IBA1 Polyclonal Antibody (Product # PA5-21274) (Red) at a dilution of 1 µg/10^6 cells. Green : isotype control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of PLK4 was performed in human monocyte using a PLK4 Polyclonal Antibody (Product # PA1-31555) (Red) at a dilution of 0.5 µg/10^6 cells. Green : isotype control. Shaded histogram : cells only.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of SR-BI/SR-BII was performed in human monocyte using 0.5 µg of SR-BI/SR-BII Polyclonal Antibody (Product # PA1-32415) (Red). Green : isotype control. Shaded histogram : cells only.
