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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Flow cytometry [2]
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- Product number
- PA5-23105 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: mouse spleen lysate or protein.
- Reactivity
- Human, Mouse, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Participation of Endosomes in Toll-Like Receptor 3 Transportation Pathway in Murine Astrocytes.
Mielcarska MB, Gregorczyk-Zboroch KP, Szulc-Da̧browska L, Bossowska-Nowicka M, Wyżewski Z, Cymerys J, Chodkowski M, Kiełbik P, Godlewski MM, Gieryńska M, Toka FN
Frontiers in cellular neuroscience 2020;14:544612
Frontiers in cellular neuroscience 2020;14:544612
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR3 in mouse spleen tissue lysate using a TLR3/CD283 polyclonal antibody (Product # PA5-23105) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR3 in mouse spleen tissue lysate. Samples were incubated in TLR3 polyclonal antibody (Product # PA5-23105) using a dilution of 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR3 in Balb/c mouse splenocytes. Samples were incubated in TLR3 polyclonal antibody (Product # PA5-23105) using a dilution of 1 µg/10^6 cells. Green represents rabbit IgG isotype control this antibody; red represents anti-TLR3 antibody (FITC conjugate).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR3 in Balb/c mouse splenocytes and mouse RAW cells. Samples were incubated in TLR3 polyclonal antibody (Product # PA5-23105) using a dilution of 1 µg/10^6 cells. Antibody (red) and isotype control antibody (green) at 1 µg/10^6 cells.
