Antibody data
- Antibody Data
- Antigen structure
- References [25]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [3]
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- Product number
- 12-9039-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD283 (TLR3) Monoclonal Antibody (TLR3.7), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The TLR3.7 monoclonal antibody reacts with human Toll-like receptor 3 (TLR3). To date, at least twelve members of the Toll-like receptor family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. In the innate immune response, TLRs recognize molecular patterns associated with microbial pathogens and induce antimicrobial activity. TLR3 recognizes double-stranded (ds)RNA, induces the activation of NF-kappaB through MyD88-dependent and -independent pathways, and the production of type I interferons (IFNs). TLR3.7 suppressed poly(I):poly(C)-mediated IFN-beta production by human fibroblasts naturally expressing TLR3 on their surface. Applications Reported: The TLR3.7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TLR3.7 antibody has been tested by intracellular staining and flow cytometric analysis of A549 and MRC5 cell lines to detect the very low level of this antigen on the surface. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- TLR3.7
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patients.
Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.
Syndrome of selective IgM deficiency with severe T cell deficiency associated with disseminated cutaneous mycobacterium avium intracellulaire infection.
Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission.
A potential mode of action for Anakinra in patients with arthrofibrosis following total knee arthroplasty.
Hepatitis C virus infection induces autocrine interferon signaling by human liver endothelial cells and release of exosomes, which inhibits viral replication.
IL-29 enhances Toll-like receptor-mediated IL-6 and IL-8 production by the synovial fibroblasts from rheumatoid arthritis patients.
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Bone marrow mononuclear cells up-regulate toll-like receptor expression and produce inflammatory mediators in response to cigarette smoke extract.
Secretion of the human Toll-like receptor 3 ectodomain is affected by single nucleotide polymorphisms and regulated by Unc93b1.
Porcine small intestinal epithelial cell line (IPEC-J2) of rotavirus infection as a new model for the study of innate immune responses to rotaviruses and probiotics.
Toll-like receptor and innate cytokine responses induced by lactobacilli colonization and human rotavirus infection in gnotobiotic pigs.
Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth.
Biochemical and functional analyses of the human Toll-like receptor 3 ectodomain.
Effects of single nucleotide polymorphisms on Toll-like receptor 3 activity and expression in cultured cells.
Toll-like receptor 2, 3, and 4 expression and function in human airway smooth muscle.
Expansion of toll-like receptor 9-expressing B cells in active systemic lupus erythematosus: implications for the induction and maintenance of the autoimmune process.
Diesel exhaust enhances virus- and poly(I:C)-induced Toll-like receptor 3 expression and signaling in respiratory epithelial cells.
Expression of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D-ligands.
Structural and functional analyses of the human Toll-like receptor 3. Role of glycosylation.
Toll-like receptor 3 is induced by and mediates antiviral activity against rhinovirus infection of human bronchial epithelial cells.
Increased expression of TLR3 in lymph nodes during simian immunodeficiency virus infection: implications for inflammation and immunodeficiency.
Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA.
17Beta-estradiol suppresses TLR3-induced cytokine and chemokine production in endometrial epithelial cells.
Activation of airway epithelial cells by toll-like receptor agonists.
Deeba E, Lambrianides A, Pantzaris M, Krashias G, Christodoulou C
BMC research notes 2020 Sep 29;13(1):460
BMC research notes 2020 Sep 29;13(1):460
Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.
Li X, Zhang B, Wang H, Zhao X, Zhang Z, Ding G, Wei F
The Journal of international medical research 2020 Jul;48(7):300060520930853
The Journal of international medical research 2020 Jul;48(7):300060520930853
Syndrome of selective IgM deficiency with severe T cell deficiency associated with disseminated cutaneous mycobacterium avium intracellulaire infection.
Gharib A, Louis AG, Agrawal S, Gupta S
American journal of clinical and experimental immunology 2015;4(2):15-27
American journal of clinical and experimental immunology 2015;4(2):15-27
Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission.
Giugliano S, Petroff MG, Warren BD, Jasti S, Linscheid C, Ward A, Kramer A, Dobrinskikh E, Sheiko MA, Gale M Jr, Golden-Mason L, Winn VD, Rosen HR
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 15;195(8):3737-47
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 15;195(8):3737-47
A potential mode of action for Anakinra in patients with arthrofibrosis following total knee arthroplasty.
Dixon D, Coates J, del Carpio Pons A, Horabin J, Walker A, Abdul N, Kalson NS, Brewster NT, Weir DJ, Deehan DJ, Mann DA, Borthwick LA
Scientific reports 2015 Nov 10;5:16466
Scientific reports 2015 Nov 10;5:16466
Hepatitis C virus infection induces autocrine interferon signaling by human liver endothelial cells and release of exosomes, which inhibits viral replication.
Giugliano S, Kriss M, Golden-Mason L, Dobrinskikh E, Stone AE, Soto-Gutierrez A, Mitchell A, Khetani SR, Yamane D, Stoddard M, Li H, Shaw GM, Edwards MG, Lemon SM, Gale M Jr, Shah VH, Rosen HR
Gastroenterology 2015 Feb;148(2):392-402.e13
Gastroenterology 2015 Feb;148(2):392-402.e13
IL-29 enhances Toll-like receptor-mediated IL-6 and IL-8 production by the synovial fibroblasts from rheumatoid arthritis patients.
Xu L, Feng X, Tan W, Gu W, Guo D, Zhang M, Wang F
Arthritis research & therapy 2013 Oct 29;15(5):R170
Arthritis research & therapy 2013 Oct 29;15(5):R170
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Harman AN, Bye CR, Nasr N, Sandgren KJ, Kim M, Mercier SK, Botting RA, Lewin SR, Cunningham AL, Cameron PU
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Bone marrow mononuclear cells up-regulate toll-like receptor expression and produce inflammatory mediators in response to cigarette smoke extract.
Zhou J, Eksioglu EA, Fortenbery NR, Chen X, Wang H, Epling-Burnette PK, Djeu JY, Wei S
PloS one 2011;6(6):e21173
PloS one 2011;6(6):e21173
Secretion of the human Toll-like receptor 3 ectodomain is affected by single nucleotide polymorphisms and regulated by Unc93b1.
Qi R, Hoose S, Schreiter J, Sawant KV, Lamb R, Ranjith-Kumar CT, Mills J, San Mateo L, Jordan JL, Kao CC
The Journal of biological chemistry 2010 Nov 19;285(47):36635-44
The Journal of biological chemistry 2010 Nov 19;285(47):36635-44
Porcine small intestinal epithelial cell line (IPEC-J2) of rotavirus infection as a new model for the study of innate immune responses to rotaviruses and probiotics.
Liu F, Li G, Wen K, Bui T, Cao D, Zhang Y, Yuan L
Viral immunology 2010 Apr;23(2):135-49
Viral immunology 2010 Apr;23(2):135-49
Toll-like receptor and innate cytokine responses induced by lactobacilli colonization and human rotavirus infection in gnotobiotic pigs.
Wen K, Azevedo MS, Gonzalez A, Zhang W, Saif LJ, Li G, Yousef A, Yuan L
Veterinary immunology and immunopathology 2009 Feb 15;127(3-4):304-15
Veterinary immunology and immunopathology 2009 Feb 15;127(3-4):304-15
Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth.
Cho WG, Albuquerque RJ, Kleinman ME, Tarallo V, Greco A, Nozaki M, Green MG, Baffi JZ, Ambati BK, De Falco M, Alexander JS, Brunetti A, De Falco S, Ambati J
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 28;106(17):7137-42
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 28;106(17):7137-42
Biochemical and functional analyses of the human Toll-like receptor 3 ectodomain.
Ranjith-Kumar CT, Miller W, Xiong J, Russell WK, Lamb R, Santos J, Duffy KE, Cleveland L, Park M, Bhardwaj K, Wu Z, Russell DH, Sarisky RT, Mbow ML, Kao CC
The Journal of biological chemistry 2007 Mar 9;282(10):7668-78
The Journal of biological chemistry 2007 Mar 9;282(10):7668-78
Effects of single nucleotide polymorphisms on Toll-like receptor 3 activity and expression in cultured cells.
Ranjith-Kumar CT, Miller W, Sun J, Xiong J, Santos J, Yarbrough I, Lamb RJ, Mills J, Duffy KE, Hoose S, Cunningham M, Holzenburg A, Mbow ML, Sarisky RT, Kao CC
The Journal of biological chemistry 2007 Jun 15;282(24):17696-705
The Journal of biological chemistry 2007 Jun 15;282(24):17696-705
Toll-like receptor 2, 3, and 4 expression and function in human airway smooth muscle.
Sukkar MB, Xie S, Khorasani NM, Kon OM, Stanbridge R, Issa R, Chung KF
The Journal of allergy and clinical immunology 2006 Sep;118(3):641-8
The Journal of allergy and clinical immunology 2006 Sep;118(3):641-8
Expansion of toll-like receptor 9-expressing B cells in active systemic lupus erythematosus: implications for the induction and maintenance of the autoimmune process.
Papadimitraki ED, Choulaki C, Koutala E, Bertsias G, Tsatsanis C, Gergianaki I, Raptopoulou A, Kritikos HD, Mamalaki C, Sidiropoulos P, Boumpas DT
Arthritis and rheumatism 2006 Nov;54(11):3601-11
Arthritis and rheumatism 2006 Nov;54(11):3601-11
Diesel exhaust enhances virus- and poly(I:C)-induced Toll-like receptor 3 expression and signaling in respiratory epithelial cells.
Ciencewicki J, Brighton L, Wu WD, Madden M, Jaspers I
American journal of physiology. Lung cellular and molecular physiology 2006 Jun;290(6):L1154-63
American journal of physiology. Lung cellular and molecular physiology 2006 Jun;290(6):L1154-63
Expression of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D-ligands.
Schreiner B, Voss J, Wischhusen J, Dombrowski Y, Steinle A, Lochmüller H, Dalakas M, Melms A, Wiendl H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Jan;20(1):118-20
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Jan;20(1):118-20
Structural and functional analyses of the human Toll-like receptor 3. Role of glycosylation.
Sun J, Duffy KE, Ranjith-Kumar CT, Xiong J, Lamb RJ, Santos J, Masarapu H, Cunningham M, Holzenburg A, Sarisky RT, Mbow ML, Kao C
The Journal of biological chemistry 2006 Apr 21;281(16):11144-51
The Journal of biological chemistry 2006 Apr 21;281(16):11144-51
Toll-like receptor 3 is induced by and mediates antiviral activity against rhinovirus infection of human bronchial epithelial cells.
Hewson CA, Jardine A, Edwards MR, Laza-Stanca V, Johnston SL
Journal of virology 2005 Oct;79(19):12273-9
Journal of virology 2005 Oct;79(19):12273-9
Increased expression of TLR3 in lymph nodes during simian immunodeficiency virus infection: implications for inflammation and immunodeficiency.
Sanghavi SK, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5314-23
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5314-23
Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA.
Jorgenson RL, Young SL, Lesmeister MJ, Lyddon TD, Misfeldt ML
Human immunology 2005 May;66(5):469-82
Human immunology 2005 May;66(5):469-82
17Beta-estradiol suppresses TLR3-induced cytokine and chemokine production in endometrial epithelial cells.
Lesmeister MJ, Jorgenson RL, Young SL, Misfeldt ML
Reproductive biology and endocrinology : RB&E 2005 Dec 29;3:74
Reproductive biology and endocrinology : RB&E 2005 Dec 29;3:74
Activation of airway epithelial cells by toll-like receptor agonists.
Sha Q, Truong-Tran AQ, Plitt JR, Beck LA, Schleimer RP
American journal of respiratory cell and molecular biology 2004 Sep;31(3):358-64
American journal of respiratory cell and molecular biology 2004 Sep;31(3):358-64
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of permeabilized (left) and non-permeabilized (right) A549 cells with 0.5 µg of Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (open histogram) or 0.5 µg of Anti-Human CD283 (TLR3) PE (filled histogram). Total cells were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Interleukin-1 receptor 1 is highly expressed on infra-patellar pad derived fibroblasts. Fibroblasts isolated from the infra-patellar fat pad and synovial membrane (both n = 5) were cultured in vitro and the relative gene expression of TLR1-10, RIG-1, RAGE and IL-1R1 quantified by qRT-PCR. ( a ) TLR expression was restricted mainly to TLR3 and TLR4 with little or no expression of other TLRs. ( b ) There was no detectable expression of RAGE but RIG-1 was expressed at levels comparable to TLR3 and TLR4. In contrast IL-1R1 expression was 100-150 fold greater than other receptors. Results are normalised to GAPDH as a loading control. Data is presented as mean +- standard error of the mean. Fibroblasts isolated from the infra-patellar fat pad were investigated for the expression of TLR3, TLR4 and IL-1R1 protein by Western Blotting (n = 4) ( c ) and flow cytometry (n = 6) ( d ). Cells expressed TLR3 and TLR4 at comparable levels at the cell surface. In contrast, expression of IL-1R1 was significantly higher than other receptors (red traces). beta-actin was used as a loading control (c) . Unstained cells (filled traces) and IgG controls (black traces) were used as flow cytometry controls (d) .
- Conjugate
- Yellow dye