Antibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-9039-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD283 (TLR3) Monoclonal Antibody (TLR3.7), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The TLR3.7 monoclonal antibody reacts with human Toll-like receptor 3 (TLR3). To date, at least twelve members of the Toll-like receptor family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. In the innate immune response, TLRs recognize molecular patterns associated with microbial pathogens and induce antimicrobial activity. TLR3 recognizes double-stranded (ds)RNA, induces the activation of NF-kappaB through MyD88-dependent and -independent pathways, and the production of type I interferons (IFNs). TLR3.7 suppressed poly(I):poly(C)-mediated IFN-beta production by human fibroblasts naturally expressing TLR3 on their surface. Applications Reported: This TLR3.7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. (Fluorochrome conjugated TLR3.7 is recommended for use in intracellular flow cytometry.) It has also been reported in in vitro inhibition of ligand binding. For optimal detection of TLR3 with this mAb by flow cytometry, PE-conjugated or Biotin-conjugated TLR3.7 (revealed with SAv-PE cat. 12-4312) are recommended. Applications Tested: This TLR3.7 antibody has been tested by intracellular staining and flow cytometric analysis of A549 and MRC5 cell lines to detect the very low level of this antigen on the surface. This can be used at less than or equal to 2.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- TLR3.7
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references A potential mode of action for Anakinra in patients with arthrofibrosis following total knee arthroplasty.
The antimicrobial protein short palate, lung, and nasal epithelium clone 1 (SPLUNC1) is differentially modulated in eosinophilic and noneosinophilic chronic rhinosinusitis with nasal polyps.
E-cadherin interactions are required for Langerhans cell differentiation.
A consensus surface activation marker signature is partially dependent on human immunodeficiency virus type 1 Nef expression within productively infected macrophages.
Negative control of TLR3 signaling by TICAM1 down-regulation.
Cytokine modulation of TLR expression and activation in mesenchymal stromal cells leads to a proinflammatory phenotype.
Single-stranded oligonucleotides can inhibit cytokine production induced by human toll-like receptor 3.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Diesel exhaust enhances virus- and poly(I:C)-induced Toll-like receptor 3 expression and signaling in respiratory epithelial cells.
Expression of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D-ligands.
Novel role of toll-like receptor 3 in hepatitis C-associated glomerulonephritis.
Toll-like receptor 3 is induced by and mediates antiviral activity against rhinovirus infection of human bronchial epithelial cells.
Increased expression of TLR3 in lymph nodes during simian immunodeficiency virus infection: implications for inflammation and immunodeficiency.
Increased expression of TLR3 in lymph nodes during simian immunodeficiency virus infection: implications for inflammation and immunodeficiency.
Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA.
mRNA is an endogenous ligand for Toll-like receptor 3.
TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction.
Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling.
Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3.
Dixon D, Coates J, del Carpio Pons A, Horabin J, Walker A, Abdul N, Kalson NS, Brewster NT, Weir DJ, Deehan DJ, Mann DA, Borthwick LA
Scientific reports 2015 Nov 10;5:16466
Scientific reports 2015 Nov 10;5:16466
The antimicrobial protein short palate, lung, and nasal epithelium clone 1 (SPLUNC1) is differentially modulated in eosinophilic and noneosinophilic chronic rhinosinusitis with nasal polyps.
Wei Y, Xia W, Ye X, Fan Y, Shi J, Wen W, Yang P, Li H, Nasal Health Group, China (NHGC).
The Journal of allergy and clinical immunology 2014 Feb;133(2):420-8
The Journal of allergy and clinical immunology 2014 Feb;133(2):420-8
E-cadherin interactions are required for Langerhans cell differentiation.
Mayumi N, Watanabe E, Norose Y, Watari E, Kawana S, Geijtenbeek TB, Takahashi H
European journal of immunology 2013 Jan;43(1):270-80
European journal of immunology 2013 Jan;43(1):270-80
A consensus surface activation marker signature is partially dependent on human immunodeficiency virus type 1 Nef expression within productively infected macrophages.
Babu R, Brown A
Retrovirology 2013 Dec 16;10:155
Retrovirology 2013 Dec 16;10:155
Negative control of TLR3 signaling by TICAM1 down-regulation.
Tao S, Zhu L, Lee P, Lee WM, Knox K, Chen J, Di YP, Chen Y
American journal of respiratory cell and molecular biology 2012 May;46(5):660-7
American journal of respiratory cell and molecular biology 2012 May;46(5):660-7
Cytokine modulation of TLR expression and activation in mesenchymal stromal cells leads to a proinflammatory phenotype.
Romieu-Mourez R, François M, Boivin MN, Bouchentouf M, Spaner DE, Galipeau J
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7963-73
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7963-73
Single-stranded oligonucleotides can inhibit cytokine production induced by human toll-like receptor 3.
Ranjith-Kumar CT, Duffy KE, Jordan JL, Eaton-Bassiri A, Vaughan R, Hoose SA, Lamb RJ, Sarisky RT, Kao CC
Molecular and cellular biology 2008 Jul;28(14):4507-19
Molecular and cellular biology 2008 Jul;28(14):4507-19
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
Diesel exhaust enhances virus- and poly(I:C)-induced Toll-like receptor 3 expression and signaling in respiratory epithelial cells.
Ciencewicki J, Brighton L, Wu WD, Madden M, Jaspers I
American journal of physiology. Lung cellular and molecular physiology 2006 Jun;290(6):L1154-63
American journal of physiology. Lung cellular and molecular physiology 2006 Jun;290(6):L1154-63
Expression of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D-ligands.
Schreiner B, Voss J, Wischhusen J, Dombrowski Y, Steinle A, Lochmüller H, Dalakas M, Melms A, Wiendl H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Jan;20(1):118-20
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Jan;20(1):118-20
Novel role of toll-like receptor 3 in hepatitis C-associated glomerulonephritis.
Wörnle M, Schmid H, Banas B, Merkle M, Henger A, Roeder M, Blattner S, Bock E, Kretzler M, Gröne HJ, Schlöndorff D
The American journal of pathology 2006 Feb;168(2):370-85
The American journal of pathology 2006 Feb;168(2):370-85
Toll-like receptor 3 is induced by and mediates antiviral activity against rhinovirus infection of human bronchial epithelial cells.
Hewson CA, Jardine A, Edwards MR, Laza-Stanca V, Johnston SL
Journal of virology 2005 Oct;79(19):12273-9
Journal of virology 2005 Oct;79(19):12273-9
Increased expression of TLR3 in lymph nodes during simian immunodeficiency virus infection: implications for inflammation and immunodeficiency.
Sanghavi SK, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5314-23
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5314-23
Increased expression of TLR3 in lymph nodes during simian immunodeficiency virus infection: implications for inflammation and immunodeficiency.
Sanghavi SK, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5314-23
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5314-23
Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA.
Jorgenson RL, Young SL, Lesmeister MJ, Lyddon TD, Misfeldt ML
Human immunology 2005 May;66(5):469-82
Human immunology 2005 May;66(5):469-82
mRNA is an endogenous ligand for Toll-like receptor 3.
Karikó K, Ni H, Capodici J, Lamphier M, Weissman D
The Journal of biological chemistry 2004 Mar 26;279(13):12542-50
The Journal of biological chemistry 2004 Mar 26;279(13):12542-50
TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction.
Oshiumi H, Matsumoto M, Funami K, Akazawa T, Seya T
Nature immunology 2003 Feb;4(2):161-7
Nature immunology 2003 Feb;4(2):161-7
Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling.
Matsumoto M, Kikkawa S, Kohase M, Miyake K, Seya T
Biochemical and biophysical research communications 2002 May 24;293(5):1364-9
Biochemical and biophysical research communications 2002 May 24;293(5):1364-9
Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3.
Alexopoulou L, Holt AC, Medzhitov R, Flavell RA
Nature 2001 Oct 18;413(6857):732-8
Nature 2001 Oct 18;413(6857):732-8
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of permeabilized A549 cell line with 1 µg of Purified Mouse IgG1 kappa Isotype Control (Product # 14-4714-82) (open histogram) or 1 µg of Purified anti-human TLR3 (TLR3.7) (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total cells were used for analysis.