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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [5]
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- Product number
- MA5-16273 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLR3 Monoclonal Antibody (27N3D4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 27N3D4
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR3 in human intestine, human spleen, and mouse RAW lysate. Samples were incubated in TLR3 monoclonal antibody (Product # MA5-16273) using a dilution of 2 µg/mL followed by a goat anti-rat Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of TLR3 using CD283/TLR3/Toll-like Receptor 3 monoclonal antibody (Product # MA5-16273). Mouse RAW cells were probed with CD283/TLR3/Toll-like Receptor 3 monoclonal antibody (Product # MA5-16273) (red) and isotype control antibody (green) at 1 µg/10^6 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR3 in Mouse RAW cells and splenocytes. Samples were incubated in TLR3 monoclonal antibody (Product # MA5-16273) using a dilution of 1 µg/10^6 cells. Antibody (Azide Free version) (red) and isotype control antibody (green) at 1 µg/10^6 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR3 in Mouse RAW cells. Samples were incubated in TLR3 monoclonal antibody (Product # MA5-16273) using a dilution of 0.1 µg/10^6 cells. Antibody (azide free) (red) and isotype control antibody (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR3 in Mouse splenocytes. Samples were incubated in TLR3 monoclonal antibody (Product # MA5-16273) using a dilution of 1 µg/10^6 cells. Antibody (red) and isotype control antibody (green) at 1 µg/10^6 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR3 in Mouse RAW cells. Samples were incubated in TLR3 monoclonal antibody (Product # MA5-16273) using a dilution of 0.1 µg/10^6 cells. Antibody (red) and isotype control antibody (green).
