H00005927-M03

antibody from Abnova Corporation

Targeting: KDM5A JARID1A, RBBP2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005927-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005927-M03, RRID:AB_565888

Product name

JARID1A monoclonal antibody (M03), clone 1H2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant JARID1A.

Antigen sequence

KVEPEVLSTDTQTSPEPGTRMNILPKRTRRVKTQS
ESGDVSRNTELKKLQIFGAGPKVVGLAMGTKDKED
EVTRRRKVTNRSDAFNMQMRQRKGTLSVNF


Isotype

IgG

Antibody clone number

1H2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of JARID1A expression in transfected 293T cell line by JARID1A monoclonal antibody (M03), clone 1H2.Lane 1: JARID1A transfected lysate (Predicted MW: 11.11 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged JARID1A is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol