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Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- MA5-35572 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KDM5A Recombinant Rabbit Monoclonal Antibody (5B10P1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: HAYSSASKSC SQGSSTPRKQ PRKSPLVPRS LEPPVLELSP GAKAQLEELM MVGDLLEVSL DETQHIWRIL QATHPPSEDR FLHIMEDDSM EEKPLKVKGK DSSEKKRKRK LEKVEQLFGE GKQKSKELKK MDKPRKKKLK LGADKSKELN KLAKKLAKEE ERKKKKEKAA AAKVELVKES TEKKREKKVL DIPSKYDWSG AEESDDENAV CAAQNCQRPC KDKVDWVQCD GGCDEWFHQV CVGVSPEMAE NEDYICINCA KKQGPVSPGP APPPSFIMSY KLPMEDLKET S
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 5B10P1
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of KDM5A in 300 μg extracts of Hela cells. Samples were precipitated with 3 μg KDM5A Monoclonal antibody (Product # MA5-35572). Western blot was performed from the immunoprecipitate using KDM5A Monoclonal antibody (Product # MA5-35572) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP analysis of KDM5A in extracts of HCT116 cells. Samples were incubated with KDM5A Monoclonal antibody (Product # MA5-35572) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
