MA1-35678

antibody from Invitrogen Antibodies

Targeting: ABCC2 CMOAT, cMRP, DJS, MRP2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA1-35678

Provider

Invitrogen Antibodies

Product name

MRP2 Monoclonal Antibody (M2III-5)

Antibody type

Monoclonal

Antigen

Other

Description

The antibody reacts with an internal epitope of MRP2, a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines. M2III-5 was raised against a fusion protein of the bacterial maltose binding protein and rat Mrp2, containing the 202-amino acid COOH terminal end of the transporter protein. The Mab detects rat, mouse and human MRP2. M2III-5 does not cross-react with the human MDR1 P-gp, MRP1, MRP3 orMRP5 gene products.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

M2III-5

Vial size

1 mL

Concentration

250 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-MRP2 Mouse Monoclonal Antibody (Product # MA1-35678) and 175 kDa band corresponding to MRP2 was observed except in SH-SY5Y and OVACR-3, along with uncharacterized bands (*) across the cell lines tested. Membrane Enriched extracts (30 µg lysate) of Hep G2 (Lane 1), A549 (Lane 2), SH-SY5Y (Lane 3) and OVCAR-3 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) XCell SureLock™ Mini-Cell and XCell II™ Blot Module (Product # EI0002). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-MRP2 Mouse Monoclonal Antibody (Product # MA1-35678) and 175 kDa band corresponding to MRP2 was observed except in Mouse and Rat Brain, along with uncharacterized bands across tissues tested. Tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Rat Liver (Lane 2), Mouse Brain (Lane 3) and Rat Brain (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by XCell SureLock™ Mini-Cell and XCell II™ Blot Module (Product # EI0002). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). (Ref : 33:947–955, 2005).