MA1-26536

antibody from Invitrogen Antibodies

Targeting: ABCC2 CMOAT, cMRP, DJS, MRP2

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-26536

Provider

Invitrogen Antibodies

Product name

MRP2 Monoclonal Antibody (M2 III-6)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-26536 detects multidrug resistance associated protein 2 from human samples.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

M2 III-6

Vial size

250 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Hepatitis C Virus Activates a Neuregulin-Driven Circuit to Modify Surface Expression of Growth Factor Receptors of the ErbB Family.

Stindt S, Cebula P, Albrecht U, Keitel V, Schulte am Esch J, Knoefel WT, Bartenschlager R, Häussinger D, Bode JG
PloS one 2016;11(2):e0148711

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 1 HCV suppresses expression of ErbB3 and modulates the expression of EGFR and ErbB2 to various extents. For (A) and (B) the hepatoma cell lines Huh7 and Huh7.5 were cultured for 48 hours while primary human hepatocytes (PHH) were isolated and cultured for 24 hours. Subsequently (A) total mRNA was analysed for transcript abundance of EGFR, ErbB2, ErbB3 and ErbB4 by rtPCR and for (B) total protein lysates were prepared and protein expression of EGFR, ErbB2 and ErbB3 was analysed by immunoblot using specific antibodies. beta-actin or GAPDH levels were determined as loading controls. For (C) to (E) Huh cell lines harbouring the subgenomic replicon of HCV genotype 1b and the respective control cell line Huh7 cells were cultured for 48 hours and thereafter for (C) total RNA and for (D and E) total protein extracts were prepared and analysed for the abundance of EGFR, ErbB2, ErbB3 and ErbB4 transcripts (C) or for the protein levels of ErbB3 (D) as well as EGFR and ErbB2 (E). Additionally, NS3 expression was assessed for control of replication and beta-actin levels for loading control. For (F) Huh9-13 cells were cultured for 24 hours and subsequently treated with 2-C'-Methylcytidine as indicated. After an additional 48 hours total protein extracts were prepared and analysed for ErbB3 and NS3 protein levels by immunoblot. (G and H) Huh7.5 cells were infected with 1 MOI of the HCVcc strain JC1 or left un-infected for control. Total RNA or protein lysates were prepared 48 hours