PA1-4198

antibody from Invitrogen Antibodies

Targeting: NFATC2 NF-ATP, NFAT1, NFATp

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-4198

Provider

Invitrogen Antibodies

Product name

NFATC2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-4198 detects NFAT2 in human samples. NFATc2 is also known as NFAT1 or NFATp.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using NFATC2 Polyclonal Antibody (Product # PA1-4198) and a 100kDa band corresponding to NFATC2 was observed across cell lines tested except IMR32 along with an uncharacterized band (*) at ~40 kDa in the cell lines tested. Whole cell extracts (30 µg lysate) of Raji (Lane 1), Ramos (Lane 2)and IMR32 (Lane 3) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).