NB300-504

antibody from Novus Biologicals

Targeting: NFATC2 NF-ATP, NFAT1, NFATp

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Gel shift (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-504

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-504, RRID:AB_2152766

Product name

Mouse Monoclonal NFATC2/NFAT1 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects nuclear factor of activated T-cells (NFAT). This does not cross react with NFAT2 (NFATc, NFATc1).

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

STIM2 (Stromal Interaction Molecule 2)-Mediated Increase in Resting Cytosolic Free Ca²⁺ Concentration Stimulates PASMC Proliferation in Pulmonary Arterial Hypertension.

Song S, Carr SG, McDermott KM, Rodriguez M, Babicheva A, Balistrieri A, Ayon RJ, Wang J, Makino A, Yuan JX
Hypertension (Dallas, Tex. : 1979) 2018 Mar;71(3):518-529

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Ebola Virus Binding to Tim-1 on T Lymphocytes Induces a Cytokine Storm.

Younan P, Iampietro M, Nishida A, Ramanathan P, Santos RI, Dutta M, Lubaki NM, Koup RA, Katze MG, Bukreyev A
mBio 2017 Sep 26;8(5)

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Mesenchymal stem cell-conditioned media suppresses inflammation-associated overproliferation of pulmonary artery smooth muscle cells in a rat model of pulmonary hypertension.

Liu J, Han Z, Han Z, He Z
Experimental and therapeutic medicine 2016 Feb;11(2):467-475

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Iron homeostasis and pulmonary hypertension: iron deficiency leads to pulmonary vascular remodeling in the rat.

Cotroneo E, Ashek A, Wang L, Wharton J, Dubois O, Bozorgi S, Busbridge M, Alavian KN, Wilkins MR, Zhao L
Circulation research 2015 May 8;116(10):1680-90

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Inhibition of interleukin-2 gene expression by human herpesvirus 6B U54 tegument protein.

Iampietro M, Morissette G, Gravel A, Flamand L
Journal of virology 2014 Nov;88(21):12452-63

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Activation of alpha(1) -adrenergic receptors stimulate the growth of small mouse cholangiocytes via calcium-dependent activation of nuclear factor of activated T cells 2 and specificity protein 1.

Alpini G, Franchitto A, Demorrow S, Onori P, Gaudio E, Wise C, Francis H, Venter J, Kopriva S, Mancinelli R, Carpino G, Stagnitti F, Ueno Y, Han Y, Meng F, Glaser S
Hepatology (Baltimore, Md.) 2011 Feb;53(2):628-39

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Chromatin remodeling complex NURF regulates thymocyte maturation.

Landry JW, Banerjee S, Taylor B, Aplan PD, Singer A, Wu C
Genes & development 2011 Feb 1;25(3):275-86

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Interleukin (IL)-15 and IL-2 reciprocally regulate expression of the chemokine receptor CX3CR1 through selective NFAT1- and NFAT2-dependent mechanisms.

Barlic J, McDermott DH, Merrell MN, Gonzales J, Via LE, Murphy PM
The Journal of biological chemistry 2004 Nov 19;279(47):48520-34

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Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95.

Kornau HC, Schenker LT, Kennedy MB, Seeburg PH
Science (New York, N.Y.) 1995 Sep 22;269(5231):1737-40

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Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95.

Kornau HC, Schenker LT, Kennedy MB, Seeburg PH
Science (New York, N.Y.) 1995 Sep 22;269(5231):1737-40

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Both normal and cancer biopsies of deparaffinized Human spleen tissue tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Cancer biopsies of deparaffinized Human colon carcinoma tissues.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chromatin Immunoprecipitation: NFATC2/NFAT1 Antibody (25A10.D6.D2) [NB300-504] - Analysis of NFATc2 was performed using cross-linked chromatin from 1x10^6 HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes. IP was performed using a multiplex microplate Matrix ChIP assay with 1.0ul/100ul well volume of an NFATc2 monoclonal antibody. Chromatin aliquots from ~1x10^5 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify exon-1 or exon-28 of the LAMC1 gene. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of ChIP is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the rat LAMC1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by LAMC1 primers are represented by black bars. Data courtesy of the Innovators Program.