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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-90403 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VIDDESDYFA SDSNQWLSKL ERETLQKREE ELRELRHASR LSKKVTIDFA GRKILEEENS LAEYHSRLDE TIQAIANGTL NQPLTKLDRS SEEPLGVLVN PNMYQSPPQW VDHTGAASQK KAFRSSGFGL EFNSFQHQLR IQDQEFQEGF DGGWCLSVHQ PWASLLVRGI KRVEGRSWYT PHRGRLWIAA TAKKPSPQEV SELQATYRLL RGKDVEFPND YPSGCLLGCV DLIDCLSQKQ FKEQFPDISQ ESDSPFVFIC KNPQEMVVKF PIKGNPKIWK LDSKIHQGAK KGLMKQNKAV; Positive Samples: A-549, HeLa, LO2, Mouse liver, Mouse testis, Mouse brain, Rat testis, Rat brain; Cellular Location: Cytoplasm, Nucleus, centrosome, cytoskeleton, cytosol, microtubule organizing center
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.94 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ASC1 in 200 μg extracts of A-549 cells. Samples were precipitated with 3 μg ASC1 Polyclonal antibody (Product # PA5-90403). Western blot was performed from the immunoprecipitate using ASC1 Polyclonal antibody (Product # PA5-90403) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASC1 in paraffin-embedded rat heart. Samples were incubated with ASC1 Polyclonal antibody (Product # PA5-90403) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASC1 in paraffin-embedded human colon carcinoma. Samples were incubated with ASC1 Polyclonal antibody (Product # PA5-90403) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASC1 in paraffin-embedded human esophageal. Samples were incubated with ASC1 Polyclonal antibody (Product # PA5-90403) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of ASC1 was performed in 200 µg extracts of A-549 cells using ASC1 Polyclonal Antibody (Product # PA5-90403). Western blot was performed from the immunoprecipitate using ASC1 Polyclonal Antibody.
