Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [10]
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Validation data
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- Product number
- NBP1-86658 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86658, RRID:AB_11024511
- Product name
- Rabbit Polyclonal PICALM Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PICALM antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Erinacine A-enriched Hericium erinaceus mycelium ameliorates Alzheimer's disease-related pathologies in APPswe/PS1dE9 transgenic mice.
Decreasing the expression of PICALM reduces endocytosis and the activity of β-secretase: implications for Alzheimer's disease.
Modulation of PICALM Levels Perturbs Cellular Cholesterol Homeostasis.
Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance.
Lysosomal network proteins as potential novel CSF biomarkers for Alzheimer's disease.
Tsai-Teng T, Chin-Chu C, Li-Ya L, Wan-Ping C, Chung-Kuang L, Chien-Chang S, Chi-Ying HF, Chien-Chih C, Shiao YJ
Journal of biomedical science 2016 Jun 27;23(1):49
Journal of biomedical science 2016 Jun 27;23(1):49
Decreasing the expression of PICALM reduces endocytosis and the activity of β-secretase: implications for Alzheimer's disease.
Thomas RS, Henson A, Gerrish A, Jones L, Williams J, Kidd EJ
BMC neuroscience 2016 Jul 18;17(1):50
BMC neuroscience 2016 Jul 18;17(1):50
Modulation of PICALM Levels Perturbs Cellular Cholesterol Homeostasis.
Mercer JL, Argus JP, Crabtree DM, Keenan MM, Wilks MQ, Chi JT, Bensinger SJ, Lavau CP, Wechsler DS
PloS one 2015;10(6):e0129776
PloS one 2015;10(6):e0129776
Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance.
Zhao Z, Sagare AP, Ma Q, Halliday MR, Kong P, Kisler K, Winkler EA, Ramanathan A, Kanekiyo T, Bu G, Owens NC, Rege SV, Si G, Ahuja A, Zhu D, Miller CA, Schneider JA, Maeda M, Maeda T, Sugawara T, Ichida JK, Zlokovic BV
Nature neuroscience 2015 Jul;18(7):978-87
Nature neuroscience 2015 Jul;18(7):978-87
Lysosomal network proteins as potential novel CSF biomarkers for Alzheimer's disease.
Armstrong A, Mattsson N, Appelqvist H, Janefjord C, Sandin L, Agholme L, Olsson B, Svensson S, Blennow K, Zetterberg H, Kågedal K
Neuromolecular medicine 2014 Mar;16(1):150-60
Neuromolecular medicine 2014 Mar;16(1):150-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PICALM Antibody [NBP1-86658] - Analysis in human cell lines Caco-2 and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PICALM Antibody [NBP1-86658] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PICALM Antibody [NBP1-86658] - Analysis using Anti-PICALM antibody NBP1-86658 (A) shows similar pattern to independent antibody NBP1-86659 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum, duodenum, placenta and skeletal muscle using Anti-PICALM antibody NBP1-86658 (A) shows similar protein distribution across tissues to independent antibody NBP1-86659 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining in human placenta and skeletal muscle tissues using NBP1-86658 antibody. Corresponding PICALM RNA-seq data are presented for the same tissues.