HPA019061

CALM, CLTH

Antibody data

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Validation data

Product number: HPA019061
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA019061, RRID:AB_1855362
Product name: Anti-PICALM
Provider product page: Atlas Antibodies - HPA019061
Antibody type: Polyclonal
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: DIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAAS RATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQ ARLKALKEQRL
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Sample type: MOUSE, RAT

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Western blot analysis in human cell lines Caco-2 and PC-3 using Anti-PICALM antibody. Corresponding PICALM RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Western blot analysis using Anti-PICALM antibody HPA019061 (A) shows similar pattern to independent antibody HPA019053 (B).
Sample type: HUMAN
Antibody #2 product nr: HPA019053
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human duodenum shows strong positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human lymph node using Anti-PICALM antibody HPA019061.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis using Anti-PICALM antibody HPA019061.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human colon using Anti-PICALM antibody HPA019061.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney using Anti-PICALM antibody HPA019061.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human colon, kidney, lymph node and testis using Anti-PICALM antibody HPA019061 (A) shows similar protein distribution across tissues to independent antibody HPA019053 (B).
Antibody #2 product nr: HPA019053
Antibody provider: Atlas Antibodies