PA5-77430

antibody from Invitrogen Antibodies

Targeting: GRM8 GLUR8, GPRC1H, mGlu8, MGLUR8

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-77430

Provider

Invitrogen Antibodies

Product name

mGluR8 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

0.8 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6) with mGluR8 (extracellular) polyclonal antibody (Product # PA5-77430) using a dilution of 1:400.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6) with mGluR8 (extracellular) polyclonal antibody (Product # PA5-77430) using a dilution of 1:400.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of mGluR8 (extracellular) in immersion-fixed, free-floating, frozen rat brain. A) Samples were probed with mGluR8 (extracellular) polyclonal antibody (Product # PA5-77430) using a dilution of 1:100, and incubated with DAPI. B) Stained nuclei (blue). C) Merged image of Panels A and B.