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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-52267 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HCFC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: APSQVQLIKA TTNSFHVKWD EVSTVEGYLL QLSTDLPYQA ASSDSSAAPN MQGVRMDPHR QGSNNIVPNS INDTINSTKT EQPATKETSM KNKPDFKALT DSNAILYPSL ASNASNHNSH VVDMLRKNEG PHTSANVGV Highest antigen sequence identity to the following orthologs: Mouse - 65%, Rat - 60%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HCFC2 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a HCFC2 Polyclonal Antibody (Product # PA5-52267).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HCFC2 in human cell line U-2 OS shows positivity in plasma membrane & nucleus but excluded from the nucleoli. Samples were probed using a HCFC2 Polyclonal Antibody (Product # PA5-52267).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HCFC2 in human cell line U-251 MG using a HCFC2 Polyclonal Antibody (Product # PA5-52267) shows localization to nucleoplasm and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HCFC2 in human testis using a HCFC2 Polyclonal Antibody (Product # PA5-52267) shows strong cytoplasmic positivity in cells in seminiferous ducts.
