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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [43]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 21710-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#21710-1-AP, RRID:AB_10859252
- Product name
- APAF1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated APAF1 antibody (Cat. #21710-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Endosulfan induced kidney cell injury by modulating ACE2 through up-regulating miR-429 in HK-2 cells.
RCN2 promotes Nasopharyngeal carcinoma progression by curbing Calcium flow and Mitochondrial apoptosis.
Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy.
Over-expression of miR-193a-3p regulates the apoptosis of colorectal cancer cells by targeting PAK3.
Dioscin relieves diabetic nephropathy via suppressing oxidative stress and apoptosis, and improving mitochondrial quality and quantity control.
Autophagy and mitochondrial dynamics contribute to the protective effect of diosgenin against 3-MCPD induced kidney injury.
Jujuboside A ameliorates high fat diet and streptozotocin induced diabetic nephropathy via suppressing oxidative stress, apoptosis, and enhancing autophagy.
FUT2 Facilitates Autophagy and Suppresses Apoptosis via p53 and JNK Signaling in Lung Adenocarcinoma Cells.
Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca(2+) homeostasis.
Sanguinarine impairs lysosomal function and induces ROS-dependent mitophagy and apoptosis in human hepatocellular carcinoma cells.
Aloe-emodin relieves zidovudine-induced injury in neonatal rat ventricular myocytes by regulating the p90rsk/p-bad/bcl-2 signaling pathway.
Diosgenin Protects Against Kidney Injury and Mitochondrial Apoptosis Induced by 3-MCPD Through the Regulation of ER Stress, Ca(2+) Homeostasis, and Bcl2 Expression.
Antagonizing CDK8 Sensitizes Colorectal Cancer to Radiation Through Potentiating the Transcription of e2f1 Target Gene apaf1.
Mitochondrial KATP channels contribute to the protective effects of hydrogen sulfide against impairment of central chemoreception of rat offspring exposed to maternal cigarette smoke.
Dexmedetomidine Mediates Neuroglobin Up-Regulation and Alleviates the Hypoxia/Reoxygenation Injury by Inhibiting Neuronal Apoptosis in Developing Rats.
Selenoprotein T protects against cisplatin-induced acute kidney injury through suppression of oxidative stress and apoptosis.
Antitumor effects of aconitine in A2780 cells via estrogen receptor β‑mediated apoptosis, DNA damage and migration.
LNC473 Regulating APAF1 IRES-Dependent Translation via Competitive Sponging miR574 and miR15b: Implications in Colorectal Cancer.
FosB recruits KAT5 to potentiate the growth and metastasis of papillary thyroid cancer in a DPP4-dependent manner.
Melatonin suppresses ER stress-dependent proapoptotic effects via AMPK in bone mesenchymal stem cells during mitochondrial oxidative damage.
EMC6 regulates acinar apoptosis via APAF1 in acute and chronic pancreatitis.
Synergetic inhibition of daidzein and regular exercise on breast cancer in bearing-4T1 mice by regulating NK cells and apoptosis pathway.
Blocking Parkin/PINK1-mediated mitophagy sensitizes hepatocellular carcinoma cells to sanguinarine-induced mitochondrial apoptosis.
Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells.
Propofol Ameliorates H9c2 Cells Apoptosis Induced by Oxygen Glucose Deprivation and Reperfusion Injury via Inhibiting High Levels of Mitochondrial Fusion and Fission.
Dexmedetomidine protects hippocampal neurons against hypoxia/reoxygenation-induced apoptosis through activation HIF-1α/p53 signaling.
Photobiomodulation for Global Cerebral Ischemia: Targeting Mitochondrial Dynamics and Functions.
Apoptosis induced by ursodeoxycholic acid in human melanoma cells through the mitochondrial pathway.
Dioscin ameliorates intestinal ischemia/reperfusion injury via adjusting miR-351-5p/MAPK13-mediated inflammation and apoptosis.
Anti-breast-Cancer Activity Exerted by β-Sitosterol-d-glucoside from Sweet Potato via Upregulation of MicroRNA-10a and via the PI3K-Akt Signaling Pathway.
MiR-21 regulates the apoptosis of keloid fibroblasts by caspase-8 and the mitochondria-mediated apoptotic signaling pathway via targeting FasL.
Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.
The subsequent biological effects of simulated microgravity on endothelial cell growth in HUVECs.
Pro-Inflammatory CXCR3 Impairs Mitochondrial Function in Experimental Non-Alcoholic Steatohepatitis.
Apoptotic Protease Activating Factor-1 Inhibitor Mitigates Myocardial Ischemia Injury via Disturbing Procaspase-9 Recruitment by Apaf-1.
Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica.
Mitochondria-Associated Apoptosis in Human Melanoma Cells Induced by Cardanol Monoene from Cashew Nut Shell Liquid.
Phloretin induces apoptosis of human esophageal cancer via a mitochondria-dependent pathway.
Inhibition of cell growth and induction of inflammation by endosulfan in HUVEC-C cells.
Cold-inducible RNA-binding protein inhibits neuron apoptosis through the suppression of mitochondrial apoptosis.
An ESRG-interacting protein, COXII, is involved in pro-apoptosis of human embryonic stem cells.
DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway.
Protective effect of flavonoid-rich extract from Rosa laevigata Michx on cerebral ischemia-reperfusion injury through suppression of apoptosis and inflammation.
Zhang B, Liu S, Sun Y, Xu D
Toxicology 2023 Jan 15;484:153392
Toxicology 2023 Jan 15;484:153392
RCN2 promotes Nasopharyngeal carcinoma progression by curbing Calcium flow and Mitochondrial apoptosis.
Yao H, Zhang S, Xie H, Fan Y, Miao M, Zhu R, Yuan L, Gu M, You Y, You B
Cellular oncology (Dordrecht, Netherlands) 2023 Aug;46(4):1031-1048
Cellular oncology (Dordrecht, Netherlands) 2023 Aug;46(4):1031-1048
Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy.
Zhong Y, Wang L, Jin R, Liu J, Luo R, Zhang Y, Zhu L, Peng X
Nutrients 2023 Apr 30;15(9)
Nutrients 2023 Apr 30;15(9)
Over-expression of miR-193a-3p regulates the apoptosis of colorectal cancer cells by targeting PAK3.
Ma T, Li H, Yang W, Liu Q, Yan H
American journal of translational research 2022;14(2):1361-1375
American journal of translational research 2022;14(2):1361-1375
Dioscin relieves diabetic nephropathy via suppressing oxidative stress and apoptosis, and improving mitochondrial quality and quantity control.
Zhong Y, Liu J, Sun D, Guo T, Yao Y, Xia X, Shi C, Peng X
Food & function 2022 Mar 21;13(6):3660-3673
Food & function 2022 Mar 21;13(6):3660-3673
Autophagy and mitochondrial dynamics contribute to the protective effect of diosgenin against 3-MCPD induced kidney injury.
Zhong Y, Sun D, Yao Y, Liu Q, Guo T, Wang X, Peng X
Chemico-biological interactions 2022 Mar 1;355:109850
Chemico-biological interactions 2022 Mar 1;355:109850
Jujuboside A ameliorates high fat diet and streptozotocin induced diabetic nephropathy via suppressing oxidative stress, apoptosis, and enhancing autophagy.
Zhong Y, Luo R, Liu Q, Zhu J, Lei M, Liang X, Wang X, Peng X
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2022 Jan;159:112697
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2022 Jan;159:112697
FUT2 Facilitates Autophagy and Suppresses Apoptosis via p53 and JNK Signaling in Lung Adenocarcinoma Cells.
Zhang Y, Yao E, Liu Y, Zhang Y, Ding M, Liu J, Chen X, Fan S
Cells 2022 Dec 13;11(24)
Cells 2022 Dec 13;11(24)
Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca(2+) homeostasis.
Zhong Y, Jin C, Han J, Zhu J, Liu Q, Sun D, Xia X, Peng X
Cell biology and toxicology 2021 Oct;37(5):795-809
Cell biology and toxicology 2021 Oct;37(5):795-809
Sanguinarine impairs lysosomal function and induces ROS-dependent mitophagy and apoptosis in human hepatocellular carcinoma cells.
Wang J, Su Q, Wu Q, Chen K, Ullah A, Ghauri MA, Zhang Y
Archives of pharmacal research 2021 Nov;44(11):1025-1036
Archives of pharmacal research 2021 Nov;44(11):1025-1036
Aloe-emodin relieves zidovudine-induced injury in neonatal rat ventricular myocytes by regulating the p90rsk/p-bad/bcl-2 signaling pathway.
Zhao W, Yuan Y, Feng B, Sun Y, Jiang H, Zhao W, Zheng Y, Zhao L, Chen T, Bai Y, Hang P, Chen Y, Du Z
Environmental toxicology and pharmacology 2021 Jan;81:103540
Environmental toxicology and pharmacology 2021 Jan;81:103540
Diosgenin Protects Against Kidney Injury and Mitochondrial Apoptosis Induced by 3-MCPD Through the Regulation of ER Stress, Ca(2+) Homeostasis, and Bcl2 Expression.
Zhong Y, Jin C, Han J, Zhu J, Liu Q, Sun D, Xia X, Zhang Y, Peng X
Molecular nutrition & food research 2021 Aug;65(15):e2001202
Molecular nutrition & food research 2021 Aug;65(15):e2001202
Antagonizing CDK8 Sensitizes Colorectal Cancer to Radiation Through Potentiating the Transcription of e2f1 Target Gene apaf1.
Chen B, Wen P, Hu G, Gao Y, Qi X, Zhu K, Chen S, Wu L, Xu A, Zhao G
Frontiers in cell and developmental biology 2020;8:408
Frontiers in cell and developmental biology 2020;8:408
Mitochondrial KATP channels contribute to the protective effects of hydrogen sulfide against impairment of central chemoreception of rat offspring exposed to maternal cigarette smoke.
Lei F, Wang W, Fu Y, Wang J, Zheng Y
PloS one 2020;15(10):e0237643
PloS one 2020;15(10):e0237643
Dexmedetomidine Mediates Neuroglobin Up-Regulation and Alleviates the Hypoxia/Reoxygenation Injury by Inhibiting Neuronal Apoptosis in Developing Rats.
Gao Y, Zhang Y, Dong Y, Wu X, Liu H
Frontiers in pharmacology 2020;11:555532
Frontiers in pharmacology 2020;11:555532
Selenoprotein T protects against cisplatin-induced acute kidney injury through suppression of oxidative stress and apoptosis.
Huang J, Bao D, Lei CT, Tang H, Zhang CY, Su H, Zhang C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Sep;34(9):11983-11996
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Sep;34(9):11983-11996
Antitumor effects of aconitine in A2780 cells via estrogen receptor β‑mediated apoptosis, DNA damage and migration.
Wang X, Lin Y, Zheng Y
Molecular medicine reports 2020 Sep;22(3):2318-2328
Molecular medicine reports 2020 Sep;22(3):2318-2328
LNC473 Regulating APAF1 IRES-Dependent Translation via Competitive Sponging miR574 and miR15b: Implications in Colorectal Cancer.
Wu H, Hu X, Li Y, Chen Q, Sun T, Qiao Y, Qin W, Wu Z, Fu B, Zhao H, Zhang R, Wei M
Molecular therapy. Nucleic acids 2020 Sep 4;21:764-779
Molecular therapy. Nucleic acids 2020 Sep 4;21:764-779
FosB recruits KAT5 to potentiate the growth and metastasis of papillary thyroid cancer in a DPP4-dependent manner.
Du J, Fu L, Ji F, Wang C, Liu S, Qiu X
Life sciences 2020 Oct 15;259:118374
Life sciences 2020 Oct 15;259:118374
Melatonin suppresses ER stress-dependent proapoptotic effects via AMPK in bone mesenchymal stem cells during mitochondrial oxidative damage.
Fan C, Feng J, Tang C, Zhang Z, Feng Y, Duan W, Zhai M, Yan Z, Zhu L, Feng L, Zhu H, Luo E
Stem cell research & therapy 2020 Oct 15;11(1):442
Stem cell research & therapy 2020 Oct 15;11(1):442
EMC6 regulates acinar apoptosis via APAF1 in acute and chronic pancreatitis.
Tan JH, Cao RC, Zhou L, Zhou ZT, Chen HJ, Xu J, Chen XM, Jin YC, Lin JY, Qi ZC, Zeng JL, Li SJ, Luo M, Hu GD, Jin J, Zhang GW
Cell death & disease 2020 Nov 11;11(11):966
Cell death & disease 2020 Nov 11;11(11):966
Synergetic inhibition of daidzein and regular exercise on breast cancer in bearing-4T1 mice by regulating NK cells and apoptosis pathway.
Wang B, Xu H, Hu X, Ma W, Zhang J, Li Y, Yu M, Zhang Y, Li X, Ye X
Life sciences 2020 Mar 15;245:117387
Life sciences 2020 Mar 15;245:117387
Blocking Parkin/PINK1-mediated mitophagy sensitizes hepatocellular carcinoma cells to sanguinarine-induced mitochondrial apoptosis.
Su Q, Wang J, Liu F, Zhang Y
Toxicology in vitro : an international journal published in association with BIBRA 2020 Aug;66:104840
Toxicology in vitro : an international journal published in association with BIBRA 2020 Aug;66:104840
Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells.
Yang N, Li C, Li H, Liu M, Cai X, Cao F, Feng Y, Li M, Wang X
Frontiers in pharmacology 2019;10:709
Frontiers in pharmacology 2019;10:709
Propofol Ameliorates H9c2 Cells Apoptosis Induced by Oxygen Glucose Deprivation and Reperfusion Injury via Inhibiting High Levels of Mitochondrial Fusion and Fission.
Zhao L, Zhuang J, Wang Y, Zhou D, Zhao D, Zhu S, Pu J, Zhang H, Yin M, Zhao W, Wang Z, Hong J
Frontiers in pharmacology 2019;10:61
Frontiers in pharmacology 2019;10:61
Dexmedetomidine protects hippocampal neurons against hypoxia/reoxygenation-induced apoptosis through activation HIF-1α/p53 signaling.
Gao Y, Yin H, Zhang Y, Dong Y, Yang F, Wu X, Liu H
Life sciences 2019 Sep 1;232:116611
Life sciences 2019 Sep 1;232:116611
Photobiomodulation for Global Cerebral Ischemia: Targeting Mitochondrial Dynamics and Functions.
Wang R, Dong Y, Lu Y, Zhang W, Brann DW, Zhang Q
Molecular neurobiology 2019 Mar;56(3):1852-1869
Molecular neurobiology 2019 Mar;56(3):1852-1869
Apoptosis induced by ursodeoxycholic acid in human melanoma cells through the mitochondrial pathway.
Yu H, Fu QR, Huang ZJ, Lin JY, Chen QX, Wang Q, Shen DY
Oncology reports 2019 Jan;41(1):213-223
Oncology reports 2019 Jan;41(1):213-223
Dioscin ameliorates intestinal ischemia/reperfusion injury via adjusting miR-351-5p/MAPK13-mediated inflammation and apoptosis.
Zheng L, Han X, Hu Y, Zhao X, Yin L, Xu L, Qi Y, Xu Y, Han X, Liu K, Peng J
Pharmacological research 2019 Jan;139:431-439
Pharmacological research 2019 Jan;139:431-439
Anti-breast-Cancer Activity Exerted by β-Sitosterol-d-glucoside from Sweet Potato via Upregulation of MicroRNA-10a and via the PI3K-Akt Signaling Pathway.
Xu H, Li Y, Han B, Li Z, Wang B, Jiang P, Zhang J, Ma W, Zhou D, Li X, Ye X
Journal of agricultural and food chemistry 2018 Sep 19;66(37):9704-9718
Journal of agricultural and food chemistry 2018 Sep 19;66(37):9704-9718
MiR-21 regulates the apoptosis of keloid fibroblasts by caspase-8 and the mitochondria-mediated apoptotic signaling pathway via targeting FasL.
Liu Y, Ren L, Liu W, Xiao Z
Biochemistry and cell biology = Biochimie et biologie cellulaire 2018 Oct;96(5):548-555
Biochemistry and cell biology = Biochimie et biologie cellulaire 2018 Oct;96(5):548-555
Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.
Chen X, Liu C, Zhao R, Zhao P, Wu J, Zhou N, Ying M
Molecular pharmaceutics 2018 Nov 5;15(11):4974-4984
Molecular pharmaceutics 2018 Nov 5;15(11):4974-4984
The subsequent biological effects of simulated microgravity on endothelial cell growth in HUVECs.
Xu D, Guo YB, Zhang M, Sun YQ
Chinese journal of traumatology = Zhonghua chuang shang za zhi 2018 Aug;21(4):229-237
Chinese journal of traumatology = Zhonghua chuang shang za zhi 2018 Aug;21(4):229-237
Pro-Inflammatory CXCR3 Impairs Mitochondrial Function in Experimental Non-Alcoholic Steatohepatitis.
Du J, Zhang X, Han J, Man K, Zhang Y, Chu ES, Nan Y, Yu J
Theranostics 2017;7(17):4192-4203
Theranostics 2017;7(17):4192-4203
Apoptotic Protease Activating Factor-1 Inhibitor Mitigates Myocardial Ischemia Injury via Disturbing Procaspase-9 Recruitment by Apaf-1.
Wang Y, Zhang Q, Zhong L, Lin M, Luo X, Liu S, Xu P, Liu X, Zhu YZ
Oxidative medicine and cellular longevity 2017;2017:9747296
Oxidative medicine and cellular longevity 2017;2017:9747296
Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica.
Zhang S, Yao F, Jing T, Zhang M, Zhao W, Zou X, Sui L, Hou L
Gene 2017 Sep 10;628:170-179
Gene 2017 Sep 10;628:170-179
Mitochondria-Associated Apoptosis in Human Melanoma Cells Induced by Cardanol Monoene from Cashew Nut Shell Liquid.
Su WC, Lin YF, Yu XP, Wang YX, Lin XD, Su QZ, Shen DY, Chen QX
Journal of agricultural and food chemistry 2017 Jul 19;65(28):5620-5631
Journal of agricultural and food chemistry 2017 Jul 19;65(28):5620-5631
Phloretin induces apoptosis of human esophageal cancer via a mitochondria-dependent pathway.
Duan H, Wang R, Yan X, Liu H, Zhang Y, Mu D, Han J, Li X
Oncology letters 2017 Dec;14(6):6763-6768
Oncology letters 2017 Dec;14(6):6763-6768
Inhibition of cell growth and induction of inflammation by endosulfan in HUVEC-C cells.
Li S, Xu D, Guo J, Sun Y
Environmental toxicology 2016 Dec;31(12):1785-1795
Environmental toxicology 2016 Dec;31(12):1785-1795
Cold-inducible RNA-binding protein inhibits neuron apoptosis through the suppression of mitochondrial apoptosis.
Zhang HT, Xue JH, Zhang ZW, Kong HB, Liu AJ, Li SC, Xu DG
Brain research 2015 Oct 5;1622:474-83
Brain research 2015 Oct 5;1622:474-83
An ESRG-interacting protein, COXII, is involved in pro-apoptosis of human embryonic stem cells.
Shi J, Ren C, Liu H, Wang L, Zhu B, Huang W, Liu W, Liu J, Liu Y, Xia X, Xu R, Jiang X
Biochemical and biophysical research communications 2015 May 1;460(2):130-5
Biochemical and biophysical research communications 2015 May 1;460(2):130-5
DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway.
Huang W, Liu J, Feng X, Chen H, Zeng L, Huang G, Liu W, Wang L, Jia W, Chen J, Ren C
Medical oncology (Northwood, London, England) 2015 Apr;32(4):115
Medical oncology (Northwood, London, England) 2015 Apr;32(4):115
Protective effect of flavonoid-rich extract from Rosa laevigata Michx on cerebral ischemia-reperfusion injury through suppression of apoptosis and inflammation.
Zhang S, Qi Y, Xu Y, Han X, Peng J, Liu K, Sun CK
Neurochemistry international 2013 Nov;63(5):522-32
Neurochemistry international 2013 Nov;63(5):522-32
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 21710-1-AP(APAF1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-APAF1 (IP:21710-1-AP, 4ug; Detection:21710-1-AP 1:500) with HEK-293 cells lysate 2000ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human heart using 21710-1-AP(APAF1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The APAF1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human APAF1. This antibody recognizes human,mouse,rat antigen. The APAF1 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
