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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- RQ4704 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- APAF1 Antibody / Apoptotic protease-activating factor 1
- Antibody type
- Monoclonal
- Description
- This highly specific APAF1 antibody is suitable for use in Immunohistochemistry/Western blot applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- BHF-1
- Vial size
- 100 ul
- Concentration
- Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
- Storage
- Store the APAF1 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with APAF1 antibody. Predicted molecular weight ~140 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with APAF1 antibody. Predicted molecular weight ~140 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse kidney tissue with APAF1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
