Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AF563 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-1 RII Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-1 RII in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human IL-1 RII is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-1 RI, recombinant rat IL-1 Rrp2, rmIL-18 R, and rmIL-18 R beta is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P27931
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Deficiency in IL-1 Receptor Type 2 Aggravates K/BxN Serum Transfer-Induced Arthritis in Mice but Has No Impact on Systemic Inflammatory Responses.
Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3.
Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3.
Martin P, Palmer G, Rodriguez E, Seemayer CA, Palomo J, Talabot-Ayer D, Gabay C
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2916-2926
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2916-2926
Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3.
O'Connor JC, Sherry CL, Guest CB, Freund GG
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 1;178(11):6886-93
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 1;178(11):6886-93
Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3.
O'Connor JC, Sherry CL, Guest CB, Freund GG
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 1;178(11):6886-93
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 1;178(11):6886-93
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 RII in Mouse Spleen. IL-1 RII was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF563) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-1 RII in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was stained with Goat Anti-Mouse IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF563, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).