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- Antibody Data
- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- PAB9952 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9952, RRID:AB_1676790
- Product name
- IL1R2 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein corresponding to human IL1R2.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1 mL
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Increased soluble interleukin-1 type II receptor concentrations in postoperative patients and in patients with sepsis syndrome.
Binding of IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist by soluble IL-1 receptors and levels of soluble IL-1 receptors in synovial fluids.
The type II 'decoy' receptor: a novel regulatory pathway for interleukin 1.
Pruitt JH, Welborn MB, Edwards PD, Harward TR, Seeger JW, Martin TD, Smith C, Kenney JA, Wesdorp RI, Meijer S, Cuesta MA, Abouhanze A, Copeland EM 3rd, Giri J, Sims JE, Moldawer LL, Oldenburg HS
Blood 1996 Apr 15;87(8):3282-8
Blood 1996 Apr 15;87(8):3282-8
Binding of IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist by soluble IL-1 receptors and levels of soluble IL-1 receptors in synovial fluids.
Arend WP, Malyak M, Smith MF Jr, Whisenand TD, Slack JL, Sims JE, Giri JG, Dower SK
Journal of immunology (Baltimore, Md. : 1950) 1994 Nov 15;153(10):4766-74
Journal of immunology (Baltimore, Md. : 1950) 1994 Nov 15;153(10):4766-74
The type II 'decoy' receptor: a novel regulatory pathway for interleukin 1.
Colotta F, Dower SK, Sims JE, Mantovani A
Immunology today 1994 Dec;15(12):562-6
Immunology today 1994 Dec;15(12):562-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblot using IL1R2 polyclonal antibody (Cat # PAB9952).This blot shows detection of an IL1R2-GST fusion protein (~500 ng, lane 1, green, 800 nm channel).Minimal reactivity is observed against GST (data not shown).Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking, the membrane was probed with the primary antibody diluted to 1 : 1,000.Incubation was for 2 hrs at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature. Molecular weight markers are shown for size comparison (Lane M, red, 700 nm channel).IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
- Validation comment
- Western Blot (Recombinant protein)
