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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-21974 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL1R2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, H1299, IL1R2 transfected Ad293 cell, soluble IL1R2 transfected Ad293 cell, mouse brain, rat brain. Predicted reactivity: Rhesus Monkey (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL1R2 was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a IL1R2 Polyclonal Antibody (Product # PA5-21974) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IL1R2 Polyclonal Antibody (Product # PA5-21974). Sample (30 µg of whole cell lysate). A: 293T. 10% SDS PAGE. IL1R2 Polyclonal Antibody (Product # PA5-21974) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IL-1 Receptor 2/CD121b in methanol-fixed A549 cells using an IL-1 Receptor 2/CD121b polyclonal antibody (Product # PA5-21974) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A549, using IL1 Receptor 2 (Product # PA5-21974) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of IL1R2 was performed in paraffin-embedded human lung cancer tissue using IL1R2 Polyclonal Antibody (Product # PA5-21974) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of IL1R2 was performed in paraffin-embedded human colon cancer tissue using IL1R2 Polyclonal Antibody (Product # PA5-21974) at a dilution of 1:500.
