10547-1-AP

antibody from Proteintech Group

Targeting: IRF5

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

10547-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10547-1-AP, RRID:AB_2280474

Product name

IRF5 antibody

Antibody type

Polyclonal

Description

IRF5 antibody (Cat. #10547-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Neohesperidin Alleviates the Neuropathic Pain Behavior of Rats by Downregulating the P2X4 Receptor.

Wang Y, Li C, Xing J, Zhu Y, Sun M, Yin S, Liu J, Zou L, Liang S, Liu S
Neurochemical research 2023 Mar;48(3):781-790

---

Cytoplasmic localization of IRF5 induces Wnt5a/E-cadherin degradation and promotes gastric cancer cells metastasis.

Du J, Sun C, Liu J, Wang X, Zhao X, Wang Y, Ma Y, Xie H, Qi C, Wang Q, Xia T, Ye F, Zhang Y
Cancer gene therapy 2023 Jun;30(6):866-877

---

Acupuncture relieves the visceral pain of diarrhea-predominant irritable bowel syndrome rats by regulating P2X4 expression.

Tang HY, Chen XQ, Wang H, Chu HR, Zhu CF, Huang S, Zhang MT, Shen GM
American journal of translational research 2022;14(8):5563-5573

---

Ccl3 enhances docetaxel chemosensitivity in breast cancer by triggering proinflammatory macrophage polarization.

Sheng D, Ma W, Zhang R, Zhou L, Deng Q, Tu J, Chen W, Zhang F, Gao N, Dong M, Wang D, Li F, Liu Y, He X, Duan S, Zhang L, Liu T, Liu S
Journal for immunotherapy of cancer 2022 May;10(5)

---

Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling.

Orliaguet L, Ejlalmanesh T, Humbert A, Ballaire R, Diedisheim M, Julla JB, Chokr D, Cuenco J, Michieletto J, Charbit J, Lindén D, Boucher J, Potier C, Hamimi A, Lemoine S, Blugeon C, Legoix P, Lameiras S, Baudrin LG, Baulande S, Soprani A, Castelli FA, Fenaille F, Riveline JP, Dalmas E, Rieusset J, Gautier JF, Venteclef N, Alzaid F
Nature communications 2022 Aug 30;13(1):5089

---

Neutrophilic Asthma Is Associated With Smoking, High Numbers of IRF5+, and Low Numbers of IL10+ Macrophages.

Turan N, van der Veen TA, Draijer C, Fattahi F, Ten Hacken NH, Timens W, van Oosterhout AJ, van den Berge M, Melgert BN
Frontiers in allergy 2021;2:676930

---

Echinacea Purpurea Extract (cichoric Acid) Exerts an Anti-inflammatory Effect on Yak PBMCs and Regulates the TLR4 Signalling Pathway.

Xue CH, A SX, Wang MJ, Wu Q, Liu JH, Zhang LF, Wu Y, Wu H, Chai ST
Journal of veterinary research 2021 Mar;65(1):109-115

---

Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl(4)/2-AAF.

Xu Y, Xu W, Liu W, Chen G, Jiang S, Chen J, Jian X, Zhang H, Liu P, Mu Y
Pharmaceutical biology 2021 Dec;59(1):1150-1160

---

Distinct roles of PI3Kδ and PI3Kγ in a toluene diisocyanate-induced murine asthma model.

Xu C, Chen S, Deng Y, Song J, Li J, Chen X, Chang P, Yao L, Tang H
Toxicology 2021 Apr 30;454:152747

---

Corilagin Ameliorates Atherosclerosis in Peripheral Artery Disease via the Toll-Like Receptor-4 Signaling Pathway in vitro and in vivo.

Li Y, Wang Y, Chen Y, Wang Y, Zhang S, Liu P, Chen Z, Song P, Luo L, Luo Y, Dang Y, Zhao L
Frontiers in immunology 2020;11:1611

---

Prenatal smoke effect on mouse offspring Igf1 promoter methylation from fetal stage to adulthood is organ and sex specific.

Zeng Z, Meyer KF, Lkhagvadorj K, Kooistra W, Reinders-Luinge M, Xu X, Huo X, Song J, Plösch T, Hylkema MN
American journal of physiology. Lung cellular and molecular physiology 2020 Mar 1;318(3):L549-L561

---

Rotator cuff repair with biological graft augmentation causes adverse tissue outcomes.

Rashid MS, Smith RDJ, Nagra N, Wheway K, Watkins B, Snelling S, Dakin SG, Carr AJ
Acta orthopaedica 2020 Dec;91(6):782-788

---

Phosphate Groups in the Lipid A Moiety Determine the Effects of LPS on Hepatic Stellate Cells: A Role for LPS-Dephosphorylating Activity in Liver Fibrosis.

Schippers M, Post E, Eichhorn I, Langeland J, Beljaars L, Malo MS, Hodin RA, Millán JL, Popov Y, Schuppan D, Poelstra K
Cells 2020 Dec 17;9(12)

---

Silica particles disorganize the polarization of pulmonary macrophages in mice.

Zhao Y, Hao C, Bao L, Wang D, Li Y, Qu Y, Ding M, Zhao A, Yao W
Ecotoxicology and environmental safety 2020 Apr 15;193:110364

---

Sedum sarmentosum Bunge extract alleviates inflammation and kidney injury via inhibition of M1-macrophage polarization.

Lu H, Cheng S, Wu C, Zheng S, Hong W, Liu L, Bai Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2019 Sep;62:152976

---

Chronic inflammation is a feature of Achilles tendinopathy and rupture.

Dakin SG, Newton J, Martinez FO, Hedley R, Gwilym S, Jones N, Reid HAB, Wood S, Wells G, Appleton L, Wheway K, Watkins B, Carr AJ
British journal of sports medicine 2018 Mar;52(6):359-367

---

Dual role of YM1+ M2 macrophages in allergic lung inflammation.

Draijer C, Robbe P, Boorsma CE, Hylkema MN, Melgert BN
Scientific reports 2018 Mar 23;8(1):5105

---

Ibuprofen and diclofenac treatments reduce proliferation of pancreatic acinar cells upon inflammatory injury and mitogenic stimulation.

Bombardo M, Malagola E, Chen R, Rudnicka A, Graf R, Sonda S
British journal of pharmacology 2018 Jan;175(2):335-347

---

Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.

Lu H, Wu L, Liu L, Ruan Q, Zhang X, Hong W, Wu S, Jin G, Bai Y
Biochemical pharmacology 2018 Aug;154:203-212

---

Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.

Jiang Z, Song F, Li Y, Xue D, Deng G, Li M, Liu X, Wang Y
Mediators of inflammation 2017;2017:9891673

---

Tumor Suppressor p53 Stimulates the Expression of Epstein-Barr Virus Latent Membrane Protein 1.

Wang Q, Lingel A, Geiser V, Kwapnoski Z, Zhang L
Journal of virology 2017 Oct 15;91(20)

---

Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality.

Li D, De S, Li D, Song S, Matta B, Barnes BJ
Scientific reports 2016 Aug 2;6:31002

---

C1q-mediated repression of human monocytes is regulated by leukocyte-associated Ig-like receptor 1 (LAIR-1).

Son M, Diamond B
Molecular medicine (Cambridge, Mass.) 2015 Feb 5;20(1):559-68

---

Distinct macrophage phenotypes in allergic and nonallergic lung inflammation.

Robbe P, Draijer C, Borg TR, Luinge M, Timens W, Wouters IM, Melgert BN, Hylkema MN
American journal of physiology. Lung cellular and molecular physiology 2015 Feb 15;308(4):L358-67

---

Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans.

Beljaars L, Schippers M, Reker-Smit C, Martinez FO, Helming L, Poelstra K, Melgert BN
Frontiers in immunology 2014;5:430

---

Characterization of transfusion-elicited acute antibody-mediated rejection in a rat model of kidney transplantation.

Huang G, Wilson NA, Reese SR, Jacobson LM, Zhong W, Djamali A
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2014 May;14(5):1061-72

---

Characterization of macrophage phenotypes in three murine models of house-dust-mite-induced asthma.

Draijer C, Robbe P, Boorsma CE, Hylkema MN, Melgert BN
Mediators of inflammation 2013;2013:632049

---

PPE38 of Mycobacterium marinum triggers the cross-talk of multiple pathways involved in the host response, as revealed by subcellular quantitative proteomics.

Wang H, Dong D, Tang S, Chen X, Gao Q
Journal of proteome research 2013 May 3;12(5):2055-66

---

Interleukin-4-induced β-catenin regulates the conversion of macrophages to multinucleated giant cells.

Binder F, Hayakawa M, Choo MK, Sano Y, Park JM
Molecular immunology 2013 Jun;54(2):157-63

---

A study on the immunomodulation of polysaccharopeptide through the TLR4-TIRAP/MAL-MyD88 signaling pathway in PBMCs from breast cancer patients.

Wang J, Dong B, Tan Y, Yu S, Bao YX
Immunopharmacology and immunotoxicology 2013 Aug;35(4):497-504

---

Activation of interferon regulatory factor 5 by site specific phosphorylation.

Chang Foreman HC, Van Scoy S, Cheng TF, Reich NC
PloS one 2012;7(3):e33098

---

4F decreases IRF5 expression and activation in hearts of tight skin mice.

Xu H, Krolikowski JG, Jones DW, Ge ZD, Pagel PS, Pritchard KA Jr, Weihrauch D
PloS one 2012;7(12):e52046

---

Smoking during pregnancy influences the maternal immune response in mice and humans.

Prins JR, Hylkema MN, Erwich JJ, Huitema S, Dekkema GJ, Dijkstra FE, Faas MM, Melgert BN
American journal of obstetrics and gynecology 2012 Jul;207(1):76.e1-14

---

PEGylation of interleukin-10 improves the pharmacokinetic profile and enhances the antifibrotic effectivity in CCl₄-induced fibrogenesis in mice.

Mattos A, de Jager-Krikken A, de Haan M, Beljaars L, Poelstra K
Journal of controlled release : official journal of the Controlled Release Society 2012 Aug 20;162(1):84-91

---

Interferon regulatory factor 4 (IRF-4) targets IRF-5 to regulate Epstein-Barr virus transformation.

Xu D, Meyer F, Ehlers E, Blasnitz L, Zhang L
The Journal of biological chemistry 2011 May 20;286(20):18261-7

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 10547-1-AP(IRF5 antibody) at dilution of 1:800

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The IRF5 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human IRF5. This antibody recognizes human,mouse,rat antigen. The IRF5 antibody has been validated for the following applications: ELISA, WB, IHC, IF analysis.

Appendix

10547-1-AP.pdf

Show more

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 10547-1-AP(IRF5 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 10547-1-AP(IRF5 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue