Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 50-9698-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IRF5 Monoclonal Antibody (ALYSCLN), eFluor 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The ALYSCLN monoclonal antibody reacts with human IRF5, a 60-63 kDa member of the interferon regulatory factor family of transcription factors that have diverse roles in host defense against viruses. IRF5 was originally identified as an important regulator of IFN alpha and IFN beta and more recently has been shown to be regulated by these interferons. IRF5 is also involved in induction of pro-inflammatory cytokines including IL-6, IL-12 and TNF alpha in response to TLR signaling. Specifically, it has been shown that MyD88 and TRAF6 interact directly with IRF5 in response to TLR activation and this interaction results in IRF5 nuclear translocation. Additionally, IRF5 has been shown to have both p53-dependent and independent effects in regulation of cell cycle and apoptosis. Genetic polymorphisms in the IRF5 locus have been implicated in many autoimmune diseases including Sjogren's syndrome, inflammatory bowel disease, multiple sclerosis and systemic lupus erythematosus. Recently, a role for IRF5 in the polarization of classically activated (M1) macrophages has been identified. In these macrophages, IRF5 directly upregulates expression of IL-12 and IL-23 and represses IL-10. Moreover, exogenous expression of IRF5 in M2-polarized (alternatively activated) macrophages induces expression of M1-related cytokines and chemokines. IRF5 is expressed at highest levels in monocytes, macrophages and plasmacytoid dendritic cells, but is also expressed in B cells and T cells. Applications Reported: This ALYSCLN antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This ALYSCLN antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- ALYSCLN
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Mangiferin inhibits macrophage classical activation via downregulating interferon regulatory factor 5 expression.
IRF5 promotes inflammatory macrophage polarization and TH1-TH17 responses.
Contribution of IRF5 in B cells to the development of murine SLE-like disease through its transcriptional control of the IgG2a locus.
Integral role of IRF-5 in the gene induction programme activated by Toll-like receptors.
Two discrete promoters regulate the alternatively spliced human interferon regulatory factor-5 isoforms. Multiple isoforms with distinct cell type-specific expression, localization, regulation, and function.
Wei Z, Yan L, Chen Y, Bao C, Deng J, Deng J
Molecular medicine reports 2016 Aug;14(2):1091-8
Molecular medicine reports 2016 Aug;14(2):1091-8
IRF5 promotes inflammatory macrophage polarization and TH1-TH17 responses.
Krausgruber T, Blazek K, Smallie T, Alzabin S, Lockstone H, Sahgal N, Hussell T, Feldmann M, Udalova IA
Nature immunology 2011 Mar;12(3):231-8
Nature immunology 2011 Mar;12(3):231-8
Contribution of IRF5 in B cells to the development of murine SLE-like disease through its transcriptional control of the IgG2a locus.
Savitsky DA, Yanai H, Tamura T, Taniguchi T, Honda K
Proceedings of the National Academy of Sciences of the United States of America 2010 Jun 1;107(22):10154-9
Proceedings of the National Academy of Sciences of the United States of America 2010 Jun 1;107(22):10154-9
Integral role of IRF-5 in the gene induction programme activated by Toll-like receptors.
Takaoka A, Yanai H, Kondo S, Duncan G, Negishi H, Mizutani T, Kano S, Honda K, Ohba Y, Mak TW, Taniguchi T
Nature 2005 Mar 10;434(7030):243-9
Nature 2005 Mar 10;434(7030):243-9
Two discrete promoters regulate the alternatively spliced human interferon regulatory factor-5 isoforms. Multiple isoforms with distinct cell type-specific expression, localization, regulation, and function.
Mancl ME, Hu G, Sangster-Guity N, Olshalsky SL, Hoops K, Fitzgerald-Bocarsly P, Pitha PM, Pinder K, Barnes BJ
The Journal of biological chemistry 2005 Jun 3;280(22):21078-90
The Journal of biological chemistry 2005 Jun 3;280(22):21078-90
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with Anti-Human CD3 PerCP-eFluor® 710 (Product # 46-0036-42), Anti-Human CD11b FITC (Product # 11-0118-42), Anti-Human CD123 PE (Product # 12-1239-42), and Fixable Viability Dye eFluor® 780 (Product # 65-0865-14). Intracellular staining with Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or Anti-Human IRF5 eFluor® 660 (right) was performed using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Viable single cells in the CD3+ (orange histogram), CD11b+ (blue histogram), and CD123+ (purple histogram) gates were used for analysis.