Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [4]
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Validation data
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- Product number
- NB100-1092 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1092, RRID:AB_10003326
- Product name
- Goat Polyclonal IRF5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize all four reported isoforms (NP_002191.1
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality.
MyD88-dependent signaling influences fibrosis and alternative macrophage activation during Staphylococcus aureus biofilm infection.
Loss of interferon regulatory factor 5 (IRF5) expression in human ductal carcinoma correlates with disease stage and contributes to metastasis.
IRF5 is required for late-phase TNF secretion by human dendritic cells.
IRF-5 is a mediator of the death receptor-induced apoptotic signaling pathway.
Methamphetamine enhances Hepatitis C virus replication in human hepatocytes.
Three functional variants of IFN regulatory factor 5 (IRF5) define risk and protective haplotypes for human lupus.
Interferon regulatory factor 5 represses expression of the Epstein-Barr virus oncoprotein LMP1: braking of the IRF7/LMP1 regulatory circuit.
Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes.
Li D, De S, Li D, Song S, Matta B, Barnes BJ
Scientific reports 2016 Aug 2;6:31002
Scientific reports 2016 Aug 2;6:31002
MyD88-dependent signaling influences fibrosis and alternative macrophage activation during Staphylococcus aureus biofilm infection.
Hanke ML, Angle A, Kielian T
PloS one 2012;7(8):e42476
PloS one 2012;7(8):e42476
Loss of interferon regulatory factor 5 (IRF5) expression in human ductal carcinoma correlates with disease stage and contributes to metastasis.
Bi X, Hameed M, Mirani N, Pimenta EM, Anari J, Barnes BJ
Breast cancer research : BCR 2011;13(6):R111
Breast cancer research : BCR 2011;13(6):R111
IRF5 is required for late-phase TNF secretion by human dendritic cells.
Krausgruber T, Saliba D, Ryzhakov G, Lanfrancotti A, Blazek K, Udalova IA
Blood 2010 Jun 3;115(22):4421-30
Blood 2010 Jun 3;115(22):4421-30
IRF-5 is a mediator of the death receptor-induced apoptotic signaling pathway.
Hu G, Barnes BJ
The Journal of biological chemistry 2009 Jan 30;284(5):2767-77
The Journal of biological chemistry 2009 Jan 30;284(5):2767-77
Methamphetamine enhances Hepatitis C virus replication in human hepatocytes.
Ye L, Peng JS, Wang X, Wang YJ, Luo GX, Ho WZ
Journal of viral hepatitis 2008 Apr;15(4):261-70
Journal of viral hepatitis 2008 Apr;15(4):261-70
Three functional variants of IFN regulatory factor 5 (IRF5) define risk and protective haplotypes for human lupus.
Graham RR, Kyogoku C, Sigurdsson S, Vlasova IA, Davies LR, Baechler EC, Plenge RM, Koeuth T, Ortmann WA, Hom G, Bauer JW, Gillett C, Burtt N, Cunninghame Graham DS, Onofrio R, Petri M, Gunnarsson I, Svenungsson E, Rönnblom L, Nordmark G, Gregersen PK, Moser K, Gaffney PM, Criswell LA, Vyse TJ, Syvänen AC, Bohjanen PR, Daly MJ, Behrens TW, Altshuler D
Proceedings of the National Academy of Sciences of the United States of America 2007 Apr 17;104(16):6758-63
Proceedings of the National Academy of Sciences of the United States of America 2007 Apr 17;104(16):6758-63
Interferon regulatory factor 5 represses expression of the Epstein-Barr virus oncoprotein LMP1: braking of the IRF7/LMP1 regulatory circuit.
Ning S, Huye LE, Pagano JS
Journal of virology 2005 Sep;79(18):11671-6
Journal of virology 2005 Sep;79(18):11671-6
Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes.
Barnes BJ, Moore PA, Pitha PM
The Journal of biological chemistry 2001 Jun 29;276(26):23382-90
The Journal of biological chemistry 2001 Jun 29;276(26):23382-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRF5 Antibody [NB100-1092] - Human Spleen (A) and Peripheral Blood Lymphocytes (B) lysates (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRF5 Antibody [NB100-1092] - Comparative analysis of IRF5 protein expression between immortalized lymphoid cell lines by immunoblot. Endogenous IRF5 protein expression in lysates (T, THP-1; R, Ramos B cells; J, Jurkat T cells) was detecting by running multiple independent blots for the analysis of antibody specificity; seven different commercially available alpha-IRF5 antibodies were evaluated. IRF5 expression was detected with the previously validated Cell Signaling antibody #3257 that is no longer available. beta-actin levels and apparent molecular weight standards are shown as loading controls and for size comparison, respectively. Arrows indicate detection of an appropriately sized band(s) corresponding to IRF5. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep31002), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRF5 Antibody [NB100-1092] - Endogenous IRF5 knockdown analysis. Ramos B cells were nucleofected with scrambled (Scr) or IRF5 (KD) siRNAs and lysates from same nucleofection run on multiple independent blots for comparative analysis of seven different commercially available alpha-IRF5 antibodies. Arrows indicate detection of an appropriately sized band(s) corresponding to IRF5. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep31002), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRF5 Antibody [NB100-1092] - Western blot analysis of Human Spleen (A) and A549 cell lysate (B) + peptide (C), 35 ug protein in RIPA buffer. Antibody at 1 ug/mL. Detected by chemiluminescence