PA5-37867

antibody from Invitrogen Antibodies

Targeting: HOXB6 HOX2, HOX2B

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-37867

Provider

Invitrogen Antibodies

Product name

HOXB6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

Evaluating the transcriptional fidelity of cancer models.

Peng D, Gleyzer R, Tai WH, Kumar P, Bian Q, Isaacs B, da Rocha EL, Cai S, DiNapoli K, Huang FW, Cahan P
Genome medicine 2021 Apr 29;13(1):73

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of HOXB6 in HEK293 lysate was performed using 35 µg protein in RIPA buffer. Lysates were probed with a HOXB6 polyclonal antibody (Product # PA5-37867) at a dilution of 1 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of HOXB6 in paraffin embedded human lung using a HOXB6 polyclonal antibody (Product #PA5-37867) at a concentration of 5 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of HOXB6 in paraffin-embedded human lung following steamed antigen retrieval with a citrate buffer, pH 6, using a HOXB6 polyclonal antibody (Product # PA5-37867) at a dilution of 5 µg/mL. Detection was performed using an AP-conjugated secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 Immunofluorescence of selected cell lines. a Classification profiles (left) and IF expression (middle) of Caov-4 (OV-positive control), HEC-59 (UCEC-positive control), and SK-OV-3 for WT1 (OV biomarker) and HOXB6 (uterine biomarker). The rightmost bar plots quantify the average percentage of positive cells for WT1 (top-right) and HOXB6 (bottom-right). b Classification profiles (left) and IF expression (middle) of Caov-4, NCCIT (germ cell tumor-positive control), and A2780 for WT1 and LIN28A (germ cell tumor biomarker). Classification was performed on replicates of NCCIT RNA-seq profiles. The rightmost bar plots quantify the average percentage of positive cells for WT1 (top-right) and LIN28A (bottom-right). c Classification profiles (left) and IF expression (middle) of Vcap (PRAD positive control), RT4 (BLCA-positive control), and PC-3 for FOLH1 (prostate biomarker) and PPARG (urothelial biomarker). The rightmost bar plots quantify the average percentage of positive cells for FOLH1 (top-right) and PPARG (bottom-right)