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LearnAF3296
antibody from Novus Biologicals
Targeting: ERCC2
EM9, MAG, MGC102762, MGC126218, MGC126219, XPD
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF3296 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal XPD Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat XPD in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references MAD2 interacts with DNA repair proteins and negatively regulates DNA damage repair.
Fung MK, Han HY, Leung SC, Cheung HW, Cheung AL, Wong YC, Ling MT, Wang X
Journal of molecular biology 2008 Aug 1;381(1):24-34
Journal of molecular biology 2008 Aug 1;381(1):24-34
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat XPD by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat XPD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3296) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPD at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
