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LearnPA5-28236
antibody from Invitrogen Antibodies
Targeting: ERCC2
EM9, MAG, MGC102762, MGC126218, MGC126219, XPD
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-28236 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- XPD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, U87-MG. Predicted reactivity: Mouse (98%), Rat (99%), Chimpanzee (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using XPD Polyclonal Antibody (Product # PA5-28236). Sample (30 µg of whole cell lysate). Lane A: IMR32. Lane B: U87-MG . 7.5% SDS PAGE. XPD Polyclonal Antibody (Product # PA5-28236) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of XPD showing staining in the nucleus of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a XPD polyclonal antibody (Product # PA5-28236) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of XPD was performed in Mock and treated HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: XPD Polyclonal Antibody (Product # PA5-28236) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERCC2 antibody [N2C2], Internal detects ERCC2 protein at cytosol and nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. ERCC2 antibody [N2C2], Internal (Product # PA5-28236) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using ERCC2 (Product # PA5-28236) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
