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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- TA800501 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA800501, RRID:AB_2625503
- Product name
- PPT1 mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)
- Antibody type
- Monoclonal
- Description
- PPT1 mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PPT1
- Isotype
- IgG
- Antibody clone number
- OTI1B10
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Reversible Cysteine Acylation Regulates the Activity of Human Palmitoyl-Protein Thioesterase 1 (PPT1).
Segal-Salto M, Sapir T, Reiner O
PloS one 2016;11(1):e0146466
PloS one 2016;11(1):e0146466
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPT1 (RC203278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPT1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
