TA319583

antibody from OriGene

Targeting: TRPC6 FSGS2, TRP6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319583

Provider

OriGene

Product name

Mouse monoclonal anti-TRPC6 antibody

Antibody type

Monoclonal

Description

Mouse monoclonal anti-TRPC6 antibody

Host

Mouse

Conjugate

Unconjugated

Epitope

TRPC6

Antibody clone number

3F2.H10.F2

Vial size

100 µg

Concentration

1mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot of Mouse anti-TRPC6 Antibody Lane 1: Mouse Kidney WCL Load: 10 ug per lane Primary antibody: TRPC6 Antibody at 1:1000 for overnight at 4°C Secondary antibody: DyLight„? 649 donkey anti-mouse at 1:20,000 for 30 min at RT Block: MB-070 for 30 min at RT

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IHC using anti-TRPC6 monoclonal antibody shows detection of TRPC6 in human adrenal (cortex) tissue (40X). The antibody was used a dilution to 2.5 ug/mL. The image shows strong staining with minimal background staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Validation comment

IHC