Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 18236-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPC6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSQSPAFGP RRGSSPRGAA GAAARRNESQ DYLLMDSELG EDGCPQAPLP CYGYYPCFRG SDNRLAHRRQ TVLREKGRRL ANRGPAYMFS DRSTSLSIEE ERFLDAAEYG NIPVVRKMLE ECHSLNVNCV DYMGQNALQL AVANEHLEIT ELLLKKENLS RVGDALLLAI SKGYVRIVEA ILSHPAFAEG KRLATSPSQS ELQQDDFYAY DEDGTRFSHD VTPIILAAHC QEYEIVHTLL RKGARIERPH DYFCKCNDCN QKQKHDSFSH SRSRINAYKG LASPAYLSLS SEDPVMTALE LSNELAVLAN IEKEFKNDYK KLSMQCKDFV VGLLDLCRNT EEVEAILNGD VETLQSGDHG RPNLSRLKLA IKYEVKKFVA HPNCQQQLLS IWYENLSGLR QQTMAV (1-405 aa encoded by B C093658)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta tissue slide using 18236-1-AP ( TRPC6 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta tissue slide using 18236-1-AP ( TRPC6 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 A549 cells were stained with 0.2ug TRPC6 antibody (18236-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-TRPC6 (IP:18236-1-AP, 4ug; Detection:18236-1-AP 1:300) with mouse lung tissue lysate 3600ug.