PA5-28100

antibody from Invitrogen Antibodies

Targeting: TRPC6 FSGS2, TRP6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28100

Provider

Invitrogen Antibodies

Product name

TRPC6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji, A549, A549 membrane fraction extract.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TRPC6 was performed by separating 30 µg of A549 whole cell and membrane extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a TRPC6 Polyclonal Antibody (Product # PA5-28100) at a dilution of 1:3000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TRPC6 Polyclonal Antibody (Product # PA5-28100). Sample (30 µg of whole cell lysate). A: HeLa S3. 7.5% SDS PAGE. TRPC6 Polyclonal Antibody (Product # PA5-28100) diluted at 1:3,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of TRPC6 was performed in paraffin-embedded mouse cerebellum tissue using Green: TRPC6 Polyclonal Antibody (Product # PA5-29847) at a dilution of 1:500. Red: NF-H, a neuron marker, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.